Pseudorabies virus protein
The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting of gI, gp50, and gp63, host cells transformed by the recombinant DNA molecule sequences, the gI, gp50 and gp63 polypeptides. The present invention also provides subunit vaccines for PRV, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals.
1. A method for distinguishing between an animal vaccinated with a Pseudorabies Virus (PRV) vaccine lacking glycoprotein gI and an unvaccinated animal without sacrificing the animal, comprising
(a) vaccinating a susceptible animal with a vaccine comprising PRV lacking glycoprotein gI;
(b) collecting serum from the vaccinated animal and the unvaccinated animal; and
(c) detecting the presence of gp50 antibodies and the absence of gI antibodies in said serum, wherein detection of gp50 antibodies and the absence of gI antibodies is indicative of a vaccinated animal.