Sequences from Piscirickettsia salmonis
The present invention relates to a fish vaccine. More specifically the invention relates to a vaccine to protect salmon against infection by Piscirickettsia salmonis . The invention is based on or derived from the nucleic acid or amino acid sequence of antigens from Piscirickettsia salmonis . Nucleic acid and/or amino acid sequences may be used in the preparation of a vaccine to protect against infection by Piscirickettsia salmonis.
1. An isolated nucleic acid sequence comprising SEQ ID NO: 33.
2. The isolated nuclei acid sequence of claim 1 whereby said isolated nucleic acid sequence is isolated from Piscirickettsia salmonis type strain LF-89.
3. An isolated nucleic acid sequence having at least 80% homology the sequence set forth in to SEQ ID NO: 33, whereby said isolated nuclei acid sequence can detect Piscirickettsia salmonis DNA by stringent hybridization conditions, wherein said stringent conditions comprise 1X SSC, 0.1% SDS at 55° C.
4. A DNA expression vector comprising a nucleic acid of claim 1 .
5. An isolated nucleic acid sequence encoding the amino acid sequence set forth in SEQ ID NO: 34.
6. An isolated polynucleotide fully complementary to the nucleic acid sequence of claim 1 .
7. A diagnostic test kit for detecting Piscirickettsia salmonis DNS comprising an isolated polynucleotide of claim 6 .
8. An isolated polynucleotide fully complementary to the nucleic acid sequence of claim 3 .