A method of forming a biosensor is provided. The method includes providing a substrate and a cover including first and second surfaces, positioning a reagent on the substrate, carving a channel by laser ablation in the first surface, and coupling the first surface of the cover to the second surface. The channel includes a first portion having a first height and a second portion having a second height that is less than the first height.
1. A method of forming a biosensor, the method comprising the steps of:
providing a substrate and a cover including first and second surfaces,
positioning a reagent on the substrate,
carving a flow channel and at least one secondary channel by laser ablation in the first surface, and
coupling the first surface of the cover to the second surface so that the flow channel is aligned with at least a portion of the reagent.
2. The method of claim 1 , wherein the cover includes opposite ends and the carving step includes carving the flow channel to extend between the ends.
3. The method of claim 2 , wherein a first portion of the channel is carved to have a first height and a second portion is carved to have a second height that is less than the first height.
4. The method of claim 3 , wherein the first portion is carved to include side walls that converge from one of the ends toward the second portion of the flow channel.
5. The method of claim 1 , further comprising the step of positioning an adhesive in the at least one secondary channel.
6. The method of claim 1 , wherein at least one secondary channel is carved in the first surface on each side of the flow channel.
7. The method of claim 6 , further comprising the step of positioning an adhesive in the secondary channels.
8. The method of claim 1 , further comprising the step of positioning electrodes on the substrate.
9. The method of claim 8 , wherein the electrodes are formed by laser ablation.
10. The method of claim 8 , wherein the first surface of the cover is coupled to the channel so that the channel extends across at least a portion of the electrodes.
11. A method of forming a biosensor, the method comprising the steps of:
providing a substrate and a cover including first and second surfaces,
positioning a reagent on the substrate,
carving a channel by laser ablation in the first surface, the channel including a first portion having a first height and a second portion having a second height that is less than the first height, and
coupling the first surface of the cover to the second surface.
12. The method of claim 11 , further comprising the step of positioning electrodes on the substrate.
13. The method of claim 12 , wherein the electrodes are formed by laser ablation.
14. The method of claim 12 , wherein the coupling step includes aligning the channel with the electrodes so that the channel extends across at least a portion of the electrodes.
15. The method of claim 11 , wherein secondary channels are carved in the cover.
16. The method of claim 11 , wherein the first portion is carved to include walls that converge toward the second portion of the channel.
17. A method of forming a biosensor, the method comprises the steps of:
providing a substrate and a cover having first and second surfaces,
applying a reagent onto the substrate to define a reaction zone,
carving a channel by laser ablation in the first surface of the cover so that the channel includes at least one floor portion and walls extending from the at least one floor portion, and
coupling the cover on the substrate so that the floor portion faces the substrate and the channel extends over at least a portion of the reagent.
18. The method of claim 17 , further comprising the step of forming an electrode set in the reaction zone.
19. The method of claim 18 , wherein the electrode set is formed by laser ablation.
20. The method of claim 17 , wherein secondary channels are carved in the first surface of the cover.