Polynucleotide
The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleoticle, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.
1. A method of treatment, comprising administering to a patient in need of such treatment an effective dose of a host cell transfected with a vector comprising a polynucleotide comprising:
a. an element comprising an extended methylation-free CpG-island;
b. an expressible gene, wherein said expressible gene is operably-linked to said CpG-island; and
c. a promoter, operably-linked to said gene, wherein said promoter is not naturally operably-linked to said CpG-island,
wherein said element facilitates reproducible activation of transcription of said gene in two or more tissue types.
2. A method of treatment, comprising administering to a patient in need of such treatment an effective dose of a host cell transfected with a vector comprising a polynucleotide comprising:
a. an element comprising an extended methylation-free CpG-island comprising at least one endogenous promoter;
b. an expressible gene, wherein said expressible gene is operably-linked to said CpG-island, further wherein said expressible gene is not naturally linked to said CpG-island; and
c. a further promoter, external to said CpG-island, operably-linked to said gene, wherein said further promoter is not naturally operably-linked to said CpG-island,
wherein said element facilitates reproducible activation of transcription of said gene in two or more tissue types.
3. A method of treatment, comprising administering to a patient in need of such treatment an effective dose of a host cell transfected with a vector comprising a polynucleotide comprising:
a. an element comprising an extended methylation-free CpG-island comprising at least one endogenous promoter;
b. an expressible gene, wherein said expressible gene is operably-linked to said CpG-island, further wherein said expressible gene is not naturally linked to said CpG-island; and
c. a further promoter, external to said CpG-island, operably-linked to said gene, wherein said further promoter is not naturally operably-linked to said CpG-island,
wherein said element facilitates reproducible activation of transcription of said gene.