Protein biopolymer markers predictive of insulin resistance
View Patent ↗The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of at least one disease state relative to recognition of the presence and/or the absence of the biopolymer, predict disease risk assessment, and develop therapeutic avenues against the disease.
1. An isolated biopolymer marker peptide consisting of SEQ ID NO: 2.
2. A method for determining the presence of an isolated biopolymer marker consisting of SEQ ID NO: 2 comprising:
(a) obtaining a sample from a patient;
(b) conducting mass spectrometric analysis on said sample to elucidate peptide fragments contained therein and comparing mass spectrum profiles of said isolated biopolymer marker consisting of SEQ ID NO:2 to mass spectrum profiles of peptides obtained and analyzed for said sample; and
(c) confirming the presence of said isolated biopolymer marker consisting of SEQ ID NO:2 in said sample displaying a peak profile at about 1189 daltons in said mass spectrum profile;
wherein the presence of said isolated biopolymer marker consisting of SEQ ID NO:2 is indicative of a link to insulin resistance.
3. The method of claim 2 , wherein said sample is an unfractionated body fluid or a tissue sample.
4. The method of claim 2 , wherein said sample is selected from the group consisting of blood, blood products, urine, saliva, cerebrospinal fluid, and lymph.
5. The method of claim 2 , wherein said mass spectrometric analysis is selected from the group consisting of Surface Enhanced Laser Desorption Ionization (SELDI) mass spectrometry (MS), Matrix-Assisted Laser Desorption/Quadrupole quadrupole Time-Of-Flight (MALDI-Og-TOF) Mass Spectrometry, Multiple Sequential Mass Spectrometry (MS/MS), Multiple Sequential Time-Of-Flight/Time-Of-Flight Mass Spectrometry (TOF-TOF) Electrospray Ionization/Ionization Time-Of-Flight Mass Spectrometry (ESI-O-TOF) and ION-TRAP Mass Spectrometry.
6. The method of claim 2 , wherein said patient is a human.
7. An insulin resistance diagnostic kit comprising: (a) an isolated biopolymer marker peptide selected from the group consisting of SEQ ID NO: 2 and (b) an antibody that binds to said isolated biopolymer marker peptide in a sample from a patient.
8. The insulin resistance diagnostic kit of claim 7 , wherein said antibody is immobilized on a solid support.
9. The insulin resistance diagnostic kit of claim 7 , wherein said antibody is labeled.