Reagents for the quantitation of active oxygen
View Patent ↗A compound represented by general formula (I) or (II) or a salt thereof and an agent for measurement of a reactive oxygen comprising said compound or a salt thereof: wherein R 1 and R 2 independently represent an aryl group which may be substituted, e.g., a phenyl group substituted with an amino group or a hydroxy group, and R 3 represents a 2-carboxyphenyl group which may be substituted.
1. A compound represented by general formula (I) or (II) or a salt thereof:
wherein R 1 and R 2 independently represent an aryl group which may be substituted and R 3 represents a 2-carboxyphenyl group which may be substituted.
2. The compound or a salt thereof according to claim 1 , wherein R 1 and R 2 represent a phenyl group substituted with an amino group or a hydroxy group.
3. The compound or a salt thereof according to claim 1 , wherein R 3 is a 2-carboxyphenyl group.
4. An agent for measurement of a reactive oxygen which comprises the compound according to claim 1 or a salt thereof.
5. A method for measurement of a reactive oxygen, which comprises the steps of:
(A) reacting the compound according to claim 1 or a salt thereof with a reactive oxygen; and
(B) measuring fluorescence of a dearylated compound or a salt thereof generated in the above step (A).
6. A method for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, by using the compound according to claim 1 or a salt thereof.
7. The method according to claim 6 , wherein the enzyme is a peroxidase.
8. An agent for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, which comprises the compound according to claim 1 or a salt thereof.
9. The agent according to claim 8 , wherein the enzyme is a peroxidase.
10. The compound or a salt thereof according to claim 2 , wherein R 3 is a 2-carboxyphenyl group.
11. An agent for measurement of a reactive oxygen which comprises the compound according to claim 2 or a salt thereof.
12. An agent for measurement of a reactive oxygen which comprises the compound according to claim 3 or a salt thereof.
13. A method for measurement of a reactive oxygen, which comprises the steps of:
(A) reacting the compound according to claim 2 or a salt thereof with a reactive oxygen; and
(B) measuring fluorescence of a dearylated compound or a salt thereof generated in the above step (A).
14. A method for measurement of a reactive oxygen, which comprises the steps of:
(A) reacting the compound according to claim 3 or a salt thereof with a reactive oxygen; and
(B) measuring fluorescence of a dearylated compound or a salt thereof generated in the above step (A).
15. A method for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, by using the compound according to claim 2 or a salt thereof.
16. A method for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, by using the compound according to claim 3 or a salt thereof.
17. An agent for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, which comprises the compound according to claim 2 or a salt thereof.
18. An agent for measurement of an enzymatic activity of an enzyme, in which a reactive oxygen involves in said enzymatic activity, which comprises the compound according to claim 3 or a salt thereof.