Transgenic animals
There is discloses a transgenic mouse over-expressing a potassium channel BEC1, which can be used as an effective tool for screening a substance for antidementia or a substance to improve learning and memory. Also disclosed are an in vivo screening method of a substance for antidementia or a substance to improve learning and memory, which uses the learning and memory abilities of said mouse as the index, and an in vivo screening of a substance for antianxiety, which uses acceleration of anxiety as the index. In addition, there is disclosed a method for producing a pharmaceutical composition for antidementia, learning and memory improvement use or antianxiety, using a substance capable of inhibiting the learning and memory potassium channel activity as the active ingredient which can be obtained by the aforementioned screening method of the present invention.
1. A transgenic mouse whose genome comprises a transgene comprising a polynucleotide encoding a potassium channel polypeptide wherein said potassium channel polypeptide comprises the sequence set forth in SEQ ID NO: 2, wherein said polynucleotide is operably linked to a neuronal cell specific promoter, wherein said potassium channel polypeptide is expressed in the brain of said transgenic mouse, and wherein said transgenic mouse exhibits reduced learning and memory abilities, dementia, or anxiety.
2. The transgenic mouse of claim 1 , wherein said promoter is an α-calcium-calmodulin-dependent kinase II promoter.
3. A method for detecting a substance that inhibits a potassium channel, said method comprising administering a candidate substance to the transgenic mouse of claim 1 , measuring anxiety in said transgenic mouse and comparing a change in anxiety to a control mouse.
4. A method for detecting a substance that inhibits anxiety, said method comprising administering a candidate substance to the transgenic mouse of claim 1 , measuring anxiety in said transgenic mouse and comparing a change in anxiety to a control mouse.
5. A method for screening a substance for anti-anxiety activity, said method comprising administering a candidate substance to the transgenic mouse of claim 1 , measuring anxiety in said transgenic mouse, and selecting a substance having an anxiety inhibiting effect.