Compositions and methods for enhancement of major histocompatibility complex class I restricted antigen presentation
Compositions and methods for eliciting an in vivo cytotoxic lymphocyte (CTL) response against a modified soluble protein antigen are disclosed. The modified soluble protein antigen comprises an added peptidic sequence which facilitates entry of the modified antigen into antigen presenting cells (APC).
1. An antigen composition capable of eliciting an enhanced cytotoxic T cell response in the context of a major histocompatibility complex class I molecule (MHC class I), comprising a soluble protein antigen modified by chemical linkage or fusion to an added peptidic sequence consisting of SEQ ID NO:4, wherein said added peptidic sequence facilitates entry of said antigen into antigen presenting cells (APC).
2. The antigen composition of claim 1 wherein said antigen is specific to a tumor or pathogen.
3. The antigen composition of claim 1 , wherein said one or more added peptidic sequences are covalently linked to said antigen.
4. The antigen composition of claim 1 wherein said antigen composition is a fusion protein produced by translation of a continuous nucleotide coding sequence.