Method and apparatus for detecting biomolecular interactions
Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.
1. A biosensor imaging system comprising:
a light source generating white light;
a collimator for collimating said white light, said collimated light being directed onto a surface of a biosensor;
an imaging spectrometer receiving reflected light from said biosensor, and
software calculating a peak wavelength value (PWV) of said reflected light.
2. The invention of claim 1 wherein said light source is a white light source.
3. The invention of claim 1 wherein said biosensor is arranged as an array of rows and columns of discrete detection areas.
4. The invention of claim 3 wherein said biosensor is coupled to a liquid containing vessel.
5. The invention of claim 1 wherein said imaging system further comprises an illuminating optical fiber coupled to the light source and wherein said illuminating optical fiber illuminates a bottom surface of said biosensor.
6. The invention of claim 1 further comprising an optical fiber for illuminating said biosensor.
7. The invention of claim 1 wherein said software enables a visual representation of said calculated PWV of said reflected light.