Preparation of enantio-specific epoxides
View Patent ↗A method for converting alkenes into epoxides and, particularly, to convert alkenes into enantio-specific epoxides by the use of enzymes which may be in their naturally-occuring (native) form or in mutated form, such as a native or mutated non-haem diiron-containing monooxygenase, and novel compounds produced thereby.
1. A method for preparing a single enantiomeric species of an epoxide comprising contacting an alkene with a native non-haem diiron-containing monooxygenase and recovering said epoxide produced.
2. The method of claim 1 wherein said monooxygenase is a toluene monooxygenase.
3. A method for preparing an epoxide comprising contacting an alkene with a mutated non-haem diiron-containing monooxygenase and recovering said epoxide produced.
4. The method of claim 3 wherein said monooxygenase is a toluene monooxygenase.
5. A method for preparing an epoxide comprising contacting an alkene with a non-haem diiron-containing monooxygenase mutated by the substitution of at least one amino acid residue.
6. The method of claim 5 wherein said monooxygenase is a toluene monooxygenase.
7. A process for producing a desired ratio of epoxide enantiomers comprising contacting an alkene with a mutated non-haem diiron monooxygenase.
8. A process for producing a desired ratio of epoxide enantiomers comprising contacting an alkene with a native non-haem diiron monooxygenase.
9. The process of claim 7 , wherein said desired ratio is 0–100:100–0 of R:S enantiomers.
10. The process of claim 8 , wherein said desired ratio is 0–100:100–0 of R:S enantiomers.