Method for tapping the immunological repertoire
View Patent ↗The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.
1. A degenerate 5′ primer that hybridizes to a region of a V H immunoglobulin domain, wherein the primer consists of 15 to 25 nucleotides and is substantially complementary to a nucleotide sequence conserved in any one of the leader or first framework regions.
2. The primer of claim 1 , wherein the region is the first framework of the variable region.
3. The primer of claim 1 , wherein the primer consists of the sequence of 5′-AGGT(C/G)(C/A)A(G/A)CT(G/T)CTCGAGTC(T/A)GG-3′.
4. The primer of claim 1 , wherein the primer comprises a nucleotide sequence defining an endonuclease recognition site.
5. A pair of primers suitable for amplifying a V H -coding region, wherein the first primer is a primer according to claim 1 , and wherein the second primer hybridizes with a nucleotide sequence conserved at the 5′ end of the non-coding strand.