PCR primer set for detecting hepatitis B virus, method for detecting hepatitis B using the primer set, and hepatitis B virus detection kit including the primer set
Provided are a primer set selected from the group consisting of primers having nucleotide sequences as set forth in SEQ ID NOS: 1–40, a method for detecting hepatitis B virus by polymerase chain reaction (PCR) using the primer set, and a hepatitis B virus detection kit including the primer set.
1. A PCR set consisting of a primer set including a primer consisting of SEQ ID NO: 4 and a primer consisting of SEQ ID NO:6.
2. A method for detecting hepatitis B virus, which comprises amplifying a nucleic acid sample obtained from an organism by PCR using the primer set of claim 1 .
3. The method of claim 2 , wherein the PCR is a two-step PCR comprising a denaturation step and an annealing and extension step.
4. The method of claim 2 , wherein the PCR is performed for 30 minutes or less.
5. The method of claim 2 , wherein the PCR is performed on a micro PCR chip.
6. The method of claim 2 , wherein the PCR is performed using 0.2-1 μM of each primer and 0.01 pg to 1 μg of a template DNA.
7. The method of claim 2 , wherein the PCR is performed under conditions of denaturation at 86-97° C. for 1-30 seconds and annealing and extension at 60-70° C. for 6-30 seconds.
8. The method of claim 5 , wherein the micro PCR chip comprises:
a silicon wafer, a surface of which is formed with a PCR chamber made by silicon lithography and the other surface is formed with a heater for heating the PCR chamber; and
a glass wafer having an inlet and an outlet.
9. A hepatitis B virus detection kit comprising the primer set of claim 1 .