Fusion protein including of CD4
Novel recombinant polypeptides are disclosed herein that include a CD4 polypeptide ligated at its C-terminus with a portion of an immunoglobulin comprising a hinge region and a constant domain of a mammalian immunoglobulin heavy chain. The portion or the IgG is fused at its C-terminus with a polypeptide comprising a tailpiece from the C-terminus of the heavy chain of an IgA antibody ara tailpiece from a C-terminus of the heavy chain of an IgM antibody. Also disclosed herein are methods for using these CD4 fusion proteins.
1. A recombinant polypeptide comprising a CD4 polypeptide ligated at its C-terminus with an immunoglobulin polypeptide, wherein the immunoglobulin polypeptide comprises a hinge region and a constant domain of a mammalian immunoglobulin heavy chain, and wherein the immunoglobulin polypeptide is fused at its C-terminus with a tailpiece polypeptide from the C terminus of the heavy chain of an IgA antibody or a tailpiece from a C terminus of the heavy chain of an IgM antibody.
2. The recombinant polypeptide of claim 1 , wherein the CD4 polypeptide comprises the D1 and D2 regions of a human CD4 polypeptide.
3. The recombinant polypeptide of claim 1 , wherein the immunoglobulin polypeptide is a human IgG molecule.
4. The recombinant polypeptide of claim 3 , wherein the imrnunoglobulin polypeptide is an IgG 1 , IgG 2 or an IgG 3 , or a combination thereof.
5. The recombinant polypeptide of claim 4 , wherein the tailpiece polypeptide comprises the tailpiece from the C terminus of the heavy chain of an IgA antibody.
6. The recombinant polypeptide of claim 1 , wherein the constant domain comprises a CH2 domain.
7. The recombinant polypeptide of claim 6 , wherein the constant domain further comprises a CH3 domain.
8. The recombinant polypeptide of claim 1 , comprising a polypeptide linker between the CD4 polypeptide and the inununoglobulin polypeptide.
9. A composition comprising the recombinant polypeptide of claim 1 in a carrier.
10. A kit for treatment or prevention of HIV infection, comprising a container comprising an effective amount of the recombinant polypeptide of claim 1 .
11. A multimeric polypeptide comprising monomers of the recombinant polypeptide of claim 5 .
12. The multimeric polypeptide of claim 11 , wherein the multimer is a dodecylmer.
13. The recombinant polypeptide of claim 5 , comprising SEQ ID NO: 1.
14. The recombinant polypeptide of claim 13 , wherein the immunoglobulin is an IgG 1 .
15. The recombinant polypeptide of claim 13 , wherein the polypeptide comprises SEQ ID NO: 6 or SEQ ID NO: 11.
16. The recombinant polypeptide of claim 13 , wherein the polypeptide consists of SEQ ID NO: 6 or SEQ ID NO: 11.