IP Library › Granted Patent US 7,374,945
Granted Patent B2
US 7,374,945 · App. 10/020,596 · Granted May 20, 2008

Method for enhancing the association rates of polynucleotides

Assignee: Gen-Probe Incorporated
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Quick Facts
Patent No.
US 7,374,945
App. No.
10/020,596
Granted
May 20, 2008
Kind
B2
Abstract

The present invention features a method and associated kit for increasing the association rate between polynucleotides having complementary, single-stranded regions, where the method includes providing to a test sample containing the complementary polynucleotides one or more synthetic, water soluble polycationic polymers.

Claims (9)

1. In a homogenous assay method for detecting in a sample a hybrid formed between a labeled polynucleotide probe and a target polynucleotide in the presence of a polycationic polymer provided to the sample in an amount sufficient to increase the rate at which the hybrid is formed, the improvement comprising providing to the sample a dissociating reagent in an amount sufficient to dissociate the polycationic polymer from the hybrid after the polynucleotide probe and the target polynucleotide have had sufficient time to associate in the sample, and detecting the hybrid in the presence of unhybridized probe after the dissociating reagent has been provided to the sample.

2. The method of claim 1 , wherein the probe polynucleotide and the polycationic polymer are in solution during the formation of the hybrid.

3. The method of claim 1 , wherein the polycationic polymer is provided to the sample before the probe polynucleotide.

4. The method of claim 1 , wherein the probe polynucleotide and the polycationic polymer are independently provided to the sample.

5. The method of claim 1 , wherein the polycationic polymer is a homopolymer.

6. The method of claim 1 , wherein the dissociating reagent is at least one of a polyanion and an anionic detergent.

7. The method of claim 6 , wherein the dissociating reagent is an anionic detergent.

8. The method of claim 7 , wherein the anionic detergent is lithium lauryl sulfate.

9. The method of claim 1 , wherein the hybrid is in solution during the detecting step.

Assignments (7)
RELEASE OF SECURITY INTEREST Recorded Apr 28, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: HOLOGIC, INC., ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO DIRECT RADIOGRAPHY CORP.; CYTYC CORPORATION, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO BIOLUCENT, LLC; CYTYC SURGICAL PRODUCTS, LLC, AS SUCCESSOR-BY-CONVERSION TO CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; GEN-PROBE INCORPORATED, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.
Reel/Frame 075566/0039 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 035820 FRAME: 0239. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY INTEREST RELEASE. Recorded Nov 9, 2017
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 044727/0529 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 028810 FRAME: 0745. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY AGREEMENT. Recorded Nov 9, 2017
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 044432/0565 →
SECURITY AGREEMENT Recorded Aug 7, 2015
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; DIRECT RADIOGRAPHY CORP.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 036307/0199 →
SECURITY INTEREST RELEASE REEL/FRAME 028810/0745 Recorded Jun 4, 2015
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 035820/0239 →
SECURITY AGREEMENT Recorded Aug 1, 2012
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 028810/0745 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 7, 2001
From: BECKER, MICHAEL M.
To: GEN-PROBE INCORPORATED
Reel/Frame 012400/0124 →
Continuity (2)
Provisional Application 6025553500 · Dec 14, 2000
Related Publication 20020164614A1 · Nov 7, 2002