Activation and expansion of cells
The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.
1. A method for activating and expanding a population of regulatory T cells by simultaneous T cell concentration and cell surface moiety ligation, comprising:
a. contacting a population of cells wherein at least a portion thereof comprises regulatory T cells with a surface, wherein said surface is a surface of a paramagnetic particle and wherein said surface has attached thereto a first agent that ligates a first T cell surface moiety of a regulatory T cell, and the same surface has attached thereto a second agent that ligates a second moiety of said regulatory T cell; and
b. applying a magnetic force that predominantly drives T cell concentration and T cell surface moiety ligation;
thereby inducing activation and expansion of said regulatory T cell.
2. The method according to claim 1 wherein the first agent is an anti-CD3 antibody or an antigen-binding fragment thereof and the second agent is an anti-CD28 antibody or an antigen-binding fragment thereof.