Plasma concentrator device
View Patent ↗A plasma concentrator of this invention having a concentrator chamber, concentrator gel beads, a filter, and an agitator. The agitator has agitator blades extending outwardly from the lower end. The agitator end is positioned in the concentrator chamber and supported for rotation about its central axis and for reciprocal movement along its central axis. The concentrator has a top with an upper opening through which the upper end of the actuator stem extends, and a lower opening in which the filter is positioned. The concentrator chamber can have a cylindrical inner wall, and the agitator blades can have an outer edge in close proximity to the inner wall with the space between the outer edge and the inner wall being less than the diameter of the gel beads. The filter is selected to block effective flow of plasma therethrough under ambient gravity conditions and permit plasma and plasma concentrate flow therethrough under centrifugal forces of the separation gravity. The method concentrates plasma by removing water without significantly denaturing the fibrinogen in the plasma. The plasma is introduced into a concentration chamber containing a plurality of dehydrated concentrator gel beads and an agitator. Then water is removed from the plasma while stirring the beads to reduce plasma polarization and breaking up clumps of beads that form during the agitation. Then centrifugal force can be applied to the concentrated plasma in an amount sufficient to separate a substantial portion of the plasma concentrate from the beads.
1. A plasma concentrator comprising a concentrator chamber, a plurality of concentrator gel beads in the concentrator chamber, a filter, and an agitator,
the agitator comprising an actuator stem having an upper agitator end and a lower agitator end, agitator blades extending outwardly from the lower end, the lower agitator end being positioned in the concentrator chamber and supported for rotation about its central axis and for reciprocal movement along its central axis, and
the concentrator having a top with an upper opening through which the upper end of the actuator stem extends, and a lower opening in which the filter is positioned.
2. The plasma concentrator of claim 1 wherein the concentrator chamber has a cylindrical inner wall, agitator blades having an outer edge in close proximity to the inner wall with the space between the outer edge and the inner wall being less than the diameter of the gel beads.
3. The plasma concentrator of claim 1 wherein the upper opening of the concentrator includes a stop sleeve extending from the top of the concentrator into the concentrator chamber, the stop sleeve having a lower abutment surface, and the agitator stem has stop projections that extend outward beyond the diameter of the strop sleeve, the upper surfaces of the stop projections constituting abutment surfaces positioned to stop upward axial movement of the agitator when contacting the lower abutment surface of the stop sleeve.
4. The plasma concentrator of claim 3 wherein the filter has an upper surface and the agitator blades have a lower portion that contacts the upper surface of the filter and is positioned for sweeping the upper surface during rotation of the agitator and for making impact with the upper surface during downward movement of the agitator along its central axis, the downward motion of the blades during the reciprocal movement of the agitator being arrested by abutment with the upper surface of the filter.
5. The plasma concentrator of claim 1 wherein the filter blocks effective flow of plasma therethrough under ambient gravity conditions and permits free plasma concentrate flow therethrough under centrifugal forces at the separation gravity.
6. The plasma concentrator of claim 5 wherein the separation gravity is greater than 10 g's.
7. The plasma concentrator of claim 1 wherein the plasma concentrator is combined with a plasma concentrate outlet conduit and a plasma concentrate reservoir with an upper opening in communication with the filter and positioned to receive plasma concentrate passing through the filter, the plasma concentrator having a sloping floor and a sump at the lowest end of the floor, one end of the plasma concentrate outlet conduit communicating with the sump.