Albumin fusion proteins
View Patent ↗The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention.
1. An albumin fusion protein comprising an interferon alpha polypeptide fused to an albumin polypeptide wherein said albumin fusion protein is produced from an isolated host cell comprising Construct ID 3165.
2. The albumin fusion protein of claim 1 , which is glycosylated.
3. The albumin fusion protein of claim 1 , which is non-glycosylated.
4. The albumin fusion protein of claim 1 , wherein said isolated host cell is yeast.
5. The albumin fusion protein of claim 4 , wherein said yeast is a Saccharomyces cerevisiae.
6. The albumin fusion protein of claim 4 , wherein said yeast is glycosylation deficient.
7. The albumin fusion protein of claim 4 , wherein said yeast is glycosylation and protease deficient.
8. The albumin fusion protein of claim 1 , wherein said isolated host cell is a mammalian cell.
9. The albumin fusion protein of claim 8 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
10. The albumin fusion protein of claim 1 , wherein the albumin fusion protein further comprises a secretion leader sequence.
11. A composition comprising the albumin fusion protein of claim 1 and a pharmaceutically acceptable carrier.
12. An albumin fusion protein comprising an interferon alpha polypeptide fused to an albumin polypeptide, wherein said albumin fusion protein is produced from an isolated host cell comprising the construct contained in ATCC Deposit No. PTA-4670.
13. The albumin fusion protein of claim 12 , which is glycosylated.
14. The albumin fusion protein of claim 12 , which is non-glycosylated.
15. The albumin fusion protein of claim 12 , wherein said isolated host cell is yeast.
16. The albumin fusion protein of claim 15 , wherein said yeast is Saccharomyces cerevisiae.
17. The albumin fusion protein of claim 15 , wherein said yeast is glycosylation deficient.
18. The albumin fusion protein of claim 15 , wherein said yeast is glycosylation and protease deficient.
19. The albumin fusion protein of claim 12 , wherein said isolated host cell is a mammalian cell.
20. The albumin fusion protein of claim 19 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
21. A composition comprising the albumin fusion protein of claim 12 and a pharmaceutically acceptable carrier.
22. An albumin fusion protein produced from an isolated host cell comprising a construct encoding an interferon alpha polypeptide fused to an albumin polypeptide, wherein said albumin fusion protein comprises amino acids 25 to 774 of SEQ ID NO:1300.
23. The albumin fusion protein of claim 22 , wherein said albumin fusion protein further comprises a secretion leader sequence.
24. The albumin fusion protein of claim 22 , which is glycosylated.
25. The albumin fusion protein of claim 22 , which is non-glycosylated.
26. The albumin fusion protein of claim 22 , wherein said isolated host cell is yeast.
27. The albumin fusion protein of claim 26 , wherein said yeast is Saccharomyces cerevisiae.
28. The albumin fusion protein of claim 26 , wherein said yeast is glycosylation deficient.
29. The albumin fusion protein of claim 26 , wherein said yeast is glycosylation and protease deficient.
30. The albumin fusion protein of claim 22 , wherein said isolated host cell is a mammalian cell.
31. The albumin fusion protein of claim 30 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
32. A composition comprising the albumin fusion protein of claim 22 and a pharmaceutically acceptable carrier.
33. A nucleic acid molecule comprising a polynucleotide encoding the albumin fusion protein of claim 1 .
34. The nucleic acid molecule of claim 33 , which encodes the albumin fusion protein further comprising an N-terminal methionine.
35. The nucleic acid molecule of claim 33 , which encodes the albumin fusion protein further comprising a signal sequence.
36. The nucleic acid molecule of claim 33 , which comprises a heterologous polynucleotide.
37. The nucleic acid molecule of claim 36 , wherein said heterologous polynucleotide is at least one selected from a vector sequence, a promoter sequence, a selectable marker, and a region for termination of transcription.
38. The nucleic acid molecule of claim 33 , which is part of an expression cassette.
39. A vector comprising the nucleic acid molecule of claim 33 .
40. An isolated host cell comprising the nucleic acid molecule of claim 33 .
41. An isolated host cell comprising the vector of claim 39 .
42. A method of producing an albumin fusion protein comprising:
(a) culturing the isolated host cell of claim 40 in conditions such that the albumin fusion protein is expressed; and
(b) recovering the albumin fusion protein.
43. The albumin fusion protein produced by the method of claim 42 .
44. A nucleic acid molecule comprising a polynucleotide encoding the albumin fusion protein of claim 12 .
45. The nucleic acid molecule of claim 44 , which encodes the albumin fusion protein further comprising an N-terminal methionine.
46. The nucleic acid molecule of claim 44 , which encodes the albumin fusion protein further comprising a signal sequence.
47. The nucleic acid molecule of claim 44 , which comprises a heterologous polynucleotide.
48. The nucleic acid molecule of claim 47 , wherein said heterologous polynucleotide is at least one selected from a vector sequence, a promoter sequence, a selectable marker, and a region for termination of transcription.
49. The nucleic acid molecule of claim 44 , which is part of an expression cassette.
50. A vector comprising the nucleic acid molecule of claim 44 .
51. An isolated host cell comprising the nucleic acid molecule of claim 44 .
52. An isolated host cell comprising the vector of claim 50 .
53. A method of producing an albumin fusion protein comprising:
(a) culturing the isolated host cell of claim 51 in conditions such that the albumin fusion protein is expressed; and
(b) recovering the albumin fusion protein.
54. The albumin fusion protein produced by the method of claim 53 .
55. A nucleic acid molecule comprising a polynucleotide encoding the albumin fusion protein of claim 22 .
56. The nucleic acid molecule of claim 55 , which encodes the albumin fusion protein further comprising an N-terminal methionine.
57. The nucleic acid molecule of claim 55 , which encodes the albumin fusion protein further comprising a signal sequence.
58. The nucleic acid molecule of claim 55 , which comprises a heterologous polynucleotide.
59. The nucleic acid molecule of claim 58 , wherein said heterologous polynucleotide is at least one selected from a vector sequence, a promoter sequence, a selectable marker, and a region for termination of transcription.
60. The nucleic acid molecule of claim 55 , which is part of an expression cassette.
61. A vector comprising the nucleic acid molecule of claim 55 .
62. An isolated host cell comprising the nucleic acid molecule of claim 55 .
63. An isolated host cell comprising the vector of claim 61 .
64. A method of producing an albumin fusion protein comprising:
(a) culturing the isolated host cell of claim 62 in conditions such that the albumin fusion protein is expressed; and
(b) recovering the albumin fusion protein.
65. The albumin fusion protein produced by the method of claim 64 .
66. The isolated host cell of claim 40 , which is prokaryotic.
67. The isolated host cell of claim 40 , which is eukaryotic.
68. The isolated host cell of claim 67 , which is a yeast cell.
69. The isolated host cell of claim 68 , wherein said yeast cell is Saccharomyces cerevisiae.
70. The isolated host cell of claim 67 , which is a mammalian cell.
71. The isolated host cell of claim 70 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
72. The isolated host cell of claim 51 , which is prokaryotic.
73. The isolated host cell of claim 51 , which is eukaryotic.
74. The isolated host cell of claim 73 , which is a yeast cell.
75. The isolated host cell of claim 74 , wherein said yeast cell is Saccharomyces cerevisiae.
76. The isolated host cell of claim 73 , which is a mammalian cell.
77. The isolated host cell of claim 76 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
78. The isolated host cell of claim 62 , which is prokaryotic.
79. The isolated host cell of claim 62 , which is eukaryotic.
80. The isolated host cell of claim 79 , which is a yeast cell.
81. The isolated host cell of claim 80 , wherein said yeast cell is Saccharomyces cerevisiae.
82. The isolated host cell of claim 79 , which is a mammalian cell.
83. The isolated host cell of claim 82 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.