Multi-lineage progenitor cells
Fetal blood multi-lineage progenitor cells that are capable of a wide spectrum of transdifferentiation are described.
1. A method of characterizing a population of multi-lineage progenitor cells (MLPC), said method comprising: a) providing a purified population of MLPC, wherein said MLPC are positive for CD9, CD13, CD29, CD44, CD73, CD90 and CD105, and negative for CD10, CD34, CD41, CD45, Stro-1, Stage Specific Embryonic Antigen-3 (SSEA-3) and SSEA-4; and b) assessing expression in said population of MLPC of at least one mRNA selected from the group consisting of CXCR4, FLT3, CD133, ICAM2, ITGAX, TFRC, KIT, IL6R, IL7R, ITGAM, FLT3, PDGFRB, SELE, SELL, TFRC, ITGAL, ITGB2, PECAM1, ITGA2B, ITGA3, ITGA4, ITGA6, ICAM1, CD24, CD34, CD44, CD45, CD58, CD68, CD33, CD37, CD38, TERT and POU5F.
2. The method of claim 1 , wherein expression of mRNA for CXCR4, FLT3, and CD133 is assessed.
3. The method of claim 1 , wherein expression of mRNA for TERT, KIT, and POU5F is assessed.
4. The method of claim 2 or 3 , wherein expression of mRNA for CD34 is assessed.
5. The method of claim 1 , wherein said MLPC are further negative for CD2, CD3, CD4, CD5, CD7, CD8, CD14, CD15, CD16, CD19, CD20, CD22, CD33, CD36, CD38, CD61, CD62E, CD133, glycophorin-A, stem cell factor, and HLA-DR.
6. The method of claim 1 , wherein expression of at least three mRNAs from said group is assessed.