Method of purifying moxidectin through crystallization
Methods for the purification of the macrolide moxidectin result in higher purity levels than can often otherwise be obtained. The crystalline moxidectin is then used in a wide variety of pharmaceutical and veterinary applications, including the prevention, treatment and control of parasites in plants, animals and humans.
1. A method for purifying moxidectin, said method comprising:
a) providing a solution of moxidectin, wherein the moxidectin is dissolved or prepared in a first organic solvent;
b) concentrating said provided moxidectin solution such that the percentage of total solids in the concentrated moxidectin solution is about 40-50% by weight;
c) adding a second organic solvent to said concentrated moxidectin solution, wherein said second organic solvent is present in a weight ratio of 2:1 to 6:1 with respect to said first organic solvent; and said second organic solvent is a non-polar organic solvent;
d) lowering the temperature of the moxidectin solution formed in c), so as to form moxidectin crystals; and
e) recovering said moxidectin crystals.
2. The method of claim 1 , further comprising drying the recovered crystals.
3. The method of claim 1 , wherein the moxidectin dissolved or prepared in the first organic solvent is amorphous moxidectin.
4. The method of claim 1 , wherein in b) said solution is concentrated at a temperature within a range of about 40 to 50° C.
5. The method of claim 4 , wherein the temperature of the concentrated solution is cooled to a temperature within the range of about 40 to 30° C. before adding the second organic solvent.
6. The method of claim 1 , wherein in b) said solution is concentrated under vacuum.
7. The method of claim 1 , wherein in c) said second organic solvent is added under agitation.
8. The method of claim 1 , wherein in d) said temperature is lowered from about 30 to 10° C. so as to effect crystallization of said moxidectin.
9. The method of claim 8 , wherein in d) said temperature is lowered during a time period of about 2 to 12 hours.
10. The method of claim 1 , wherein said moxidectin is combined with said first organic solvent in a weight ratio of about 1:1 to 1:2.
11. The method of claim 1 , wherein said first organic solvent is selected from the group consisting of methanol, ethanol, methylcyclohexane, hexane, benzyl alcohol, toluene, heptane, or a mixture thereof.
12. The method of claim 1 , wherein said second organic solvent is selected from the group consisting of hexane, heptane, toluene, isooctane and mixtures thereof.
13. The method of claim 1 , wherein said second organic solvent is n-heptane and said first organic solvent is methylcyclohexane.
14. The method of claim 13 , wherein said n-heptane is added to said moxidectin/methylcyclohexane solution at a ratio of about 3:1-5:1 for n-heptane to methylcyclohexane.
15. The method of claim 1 , wherein the second organic solvent is n-hexane and said first organic solvent is methylcyclohexane.
16. The method of claim 15 , wherein said n-hexane is added to said moxidectin/methylcyclohexane solution at a ratio of about 3:1 for n-hexane to methylcyclohexane.
17. The method of claim 3 , wherein said amorphous moxidectin has a purity of about 90-92%.
18. The method of claim 1 , wherein said crystalline moxidectin is at least about 94-96% pure.
19. The method of claim 3 , wherein said recovered moxidectin crystals are 2-5% purer than said amorphous moxidectin.