Container with at least one electrode
View Patent ↗The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A container 20, 30 of the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.
1. Container for receiving an aqueous solution, which comprises
at least one area which acts as an electrode when an electric voltage is applied and a subsequent discharge occurs,
wherein said at least one electrode is made of a conductive synthetic material which is, or is at least based on a plastic material which is doped with at least one conductive substance,
wherein an overall concentration of said dope in said plastic material is 20-80% w/w, and
wherein the container is for electroporation or electrofusion of cells, derivatives of cells, subcellular particles and/or vesicles and is, at least partially, formed by an outer limit which forms an inner chamber for receiving said solution.
2. Container according to claim 1 , wherein said dope consists essentially of carbon fibers, graphite, soot and/or carbon nanotubes.
3. Container according to claim 1 , wherein the overall concentration of said dope in said plastic material is 20-60% w/w.
4. Container according to claim 1 , wherein the overall concentration of said dope in said plastic material is 40-80% w/w.
5. Container according to claim 1 , wherein said plastic material is polycarbonate, polyetheretherketone, polypropylene, polyamide, polyphenylensulfide or a mixture of these polymers, or at least based on one or several of these polymers, and/or wherein said plastic material is an intrinsically conductive synthetic material.
6. Container according to claim 5 , wherein said plastic material is an intrinsically conductive synthetic material and wherein said intrinsically conductive synthetic material is polyaniline, polyacetylene, poly-para-phenylene, poly-para-phenylensulfide, polypyrroles, polythiophene, polypropylene, or at least based on one or several of these polymers.
7. Container according to claim 1 , wherein said outer limit is made of synthetic material.
8. Container according to claim 7 , wherein said synthetic material is the same plastic material as the plastic material on which said at least one electrode is based.
9. Container according to claim 1 , wherein said at least one electrode is integrated into said outer limit.
10. Container according to claim 1 comprising at least two electrodes being made of the same material.
11. Container according to claim 1 comprising at least two electrodes, wherein said at least two electrodes are made of different materials.
12. Container according to claim 1 , wherein said at least one electrode is made of polyamide doped with 25-45% w/w carbon fibers and 15-35% w/w graphite.
13. Container according to claim 1 , wherein said at least one electrode is made of polyamide doped with 30-50% w/w carbon fibers and 25-45% w/w graphite.
14. Container according to claim 1 , wherein said at least one electrode is made of polycarbonate doped with 15-40% w/w carbon fibers and 1-40% w/w graphite.
15. Container according to claim 1 , wherein said at least one electrode is made of polyetheretherketone doped with 30-50% w/w carbon fibers.
16. Container according to claim 1 , wherein said at least one electrode is made of polyamide, preferably polyamide 66, doped with 20-40% w/w carbon fibers.
17. Container according to claim 1 , wherein said at least one electrode is made of polypropylene doped with 20% w/w carbon fibers.
18. Container according to claim 1 , wherein said at least one electrode is made of polyphenylensulfide doped with 30-50% w/w carbon fibers.
19. Container according to claim 1 , wherein said outer limit comprises at least one opening for supplying said solution and at least one opening for draining off said solution.
20. Container arrangement comprising at least two, preferably 6, 12, 24, 48, 96 or more, containers according to claim 1 being joined to build one unit.
21. Container according to claim 1 , wherein said aqueous solution comprises cells, derivatives of cells, subcellular particles and/or vesicles.
22. Container according to claim 7 , wherein said synthetic material is a transparent plastic material.
23. Container according to claim 3 , wherein the overall concentration of said dope in said plastic material is 40-60% w/w.
24. Container according to claim 3 , wherein the overall concentration of said dope in said plastic material is 50-60% w/w.
25. Container according to claim 4 , wherein the overall concentration of said dope in said plastic material is 50-80% w/w.
26. Container according to claim 4 , wherein the overall concentration of said dope in said plastic material is 60-80% w/w.
27. Container according to claim 4 , wherein the overall concentration of said dope in said plastic material is 70-80% w/w.
28. Container according to claim 12 , wherein said at least one electrode is made of polyamide 66 or polyamide 6.
29. Container according to claim 12 , wherein said at least one electrode is doped with 30-40% w/w-carbon fibers.
30. Container according to claim 12 , wherein said at least one electrode is doped with 33-37% w/w carbon fibers.
31. Container according to claim 12 , wherein said at least one electrode is doped with 20-30% w/w graphite.
32. Container according to claim 12 , wherein said at least one electrode is doped with 23-27% w/w graphite.
33. Container according to claim 13 , wherein said at least one electrode is made of polyamide 66 or polyamide 6.
34. Container according to claim 13 , wherein said at least one electrode is doped with 35-45% w/w carbon fibers.
35. Container according to claim 13 , wherein said at least one electrode is doped with 39-41% w/w carbon fibers.
36. Container according to claim 13 , wherein said at least one electrode is doped with 30-40% w/w graphite.
37. Container according to claim 13 , wherein said at least one electrode is doped with 34-36% w/w graphite.
38. Container according to claim 14 , wherein at least one electrode is made of polycarbonate doped with 20% w/w carbon fibers and 15% w/w graphite.
39. Container according to claim 1 , wherein said at least one electrode has a surface that is plane-parallel to a surface of a second electrode.
40. Container according to claim 1 , wherein the electrode is moldable.
41. Container according to claim 40 , wherein the electrode is injection-molded.