Use of a novel eimeria gene and corresponding protein
The invention relates to a novel oocyst sporocyst protein (EtOs22) belonging to the parasite of the species Eimeria tenella and to the polynucleotide encoding this protein, to vectors which contain this polynucleotide, to cells which are transformed with these vectors, to antibodies which are directed against the protein, to vaccines which comprise the polynucleotide, the protein, or fragments thereof, the abovementioned vectors or antibodies directed against the protein, and to the use of polynucleotide or of polypeptide for finding active compounds for treating an infection with Eimeria and active compounds which are suitable for the therapy of an infection with Eimeria.
1. An isolated polynucleotide consisting of:
a) the sequence of SEQ ID NO: 1; or
b) a polynucleotide which differs from a polynucleotide having the sequence of SEQ ID NO: 1 due to the degeneracy of the genetic code.
2. A vector or expression system which contains at least one of the polynucleotides as claimed in claim 1 .
3. A host cell which harbors the vector or the expression system as claimed in claim 2 .
4. A method for preparing a polypeptide having SEQ ID NO: 2 using the host cell as claimed in claim 3 .
5. A method for detecting a polynucleotide as claimed in claim 1 , wherein a polynucleotide as claimed in claim 1 is hybridized with the nucleic acid material from a biological sample and the hybridization is detected.
6. The method as claimed in claim 5 , wherein the hybridization is detected using the polymerase chain reaction.