Phage displayed cell binding peptides
The present disclosure relates to the construction, expression, and selection of genes that encode novel Trp cage polypeptides with desirable cell and/or tissue binding properties, as well as the novel Trp cage polypeptides themselves. A polypeptide of this disclosure may contain all or part of amino acid sequence AAADX 1 YX 2 QWLX 3 X 4 X 5 GPX 6 SGRPPPX 7 (SEQ ID NO: 4), wherein X n represents an amino acid found in position n, the polypeptide comprising a tryptophan cage (Trp cage).
1. A method for delivering a drug, compound or biologically active agent to a lung tissue of a subject, the method comprising: complexing or conjugating the drug, compound or biologically active agent with a polypeptide comprising the amino acid sequence of SEQ ID NO: 543 or SEQ ID NO: 551 thereby forming a complex or conjugate, and administering the complex or conjugate to the lung tissue of the subject.
2. The method of claim 1 , wherein the drug, compound or biologically active agent is an siRNA molecule.
3. The method of claim 1 , wherein the polypeptide binds lung tissue.
4. The method of claim 1 , wherein the polypeptide binds a human bronchial epithelial cell.
5. The method of claim 1 , wherein the complex or conjugate is administered intravenously.
6. The method of claim 1 , wherein the complex or conjugate is administered by injection.
7. A method for delivering a biologically active agent to a lung cell, the method comprising: complexing or conjugating the biologically active agent with a polypeptide comprising the amino acid sequence of SEQ ID NO: 543 or SEQ ID NO: 551, thereby forming a complex or conjugate, and administering the complex or conjugate to the lung cell.
8. The method of claim 7 , wherein the biologically active agent is an siRNA molecule.
9. The method of claim 7 , wherein the polypeptide binds the lung cell.
10. The method of claim 7 , wherein the polypeptide binds a bronchial epithelial cell.
11. The method of claim 7 , wherein the complex or conjugate is administered intravenously or by injection.
12. A method for preparing an RNAi agent for targeting to lung tissue, the method comprising:
providing an RNAi agent; and
contacting the RNAi agent with a polypeptide comprising the amino acid sequence of SEQ ID NO: 543 or SEQ ID NO: ID NO: 551, thereby forming a complex or conjugate of the RNAi agent.
13. The method of claim 12 , wherein the RNAi agent is an siRNA molecule.
14. The method of claim 12 , wherein the polypeptide binds the lung tissue.
15. The method of claim 12 , wherein the polypeptide binds a bronchial epithelial cell.