Albumin fusion proteins
View Patent ↗The present invention encompasses albumin fusion proteins. Nucleic acid molecules encodings the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the intention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention.
1. A method of treating Hepatitis, Cancer, or HIV in a patient, comprising:
administering a fusion protein comprising a human serum albumin (HSA) protein and a human interferon alpha polypeptide, wherein the HSA protein comprises the amino acid sequence of SEQ ID NO:18 and increases the serum plasma half-life of the human interferon alpha polypeptide compared with a human interferon alpha polypeptide that is not fused to the the HSA protein.
2. The method of claim 1 , wherein the fusion protein comprises a flexible peptide linker.
3. The method of claim 1 , wherein the human interferon alpha polypeptide is fused to the N-terminal end of the HSA protein.
4. The method of claim 1 , wherein the human interferon alpha polypeptide is fused to the C-terminal end of the HSA protein.
5. The method of claim 1 , wherein the human interferon alpha polypeptide is selected from:
i. human interferon alpha-2a;
ii. human interferon alpha-2b;
iii. human interferon alpha-n1; or
iv. human interferon alpha-n3.
6. The method of claim 1 , further comprising administration of ribavirin to a Hepatitis patient.
7. The method of claim 1 , wherein the fusion protein is expressed in yeast.
8. The method of claim 7 , wherein said yeast is a Saccharomyces cerevisiae.
9. The method of claim 8 , wherein said yeast is glycosylation deficient.
10. The method of claim 1 , wherein the fusion protein is expressed by a mammalian cell.
11. The method of claim 10 , wherein said mammalian cell is a COS, CHO (Chinese hamster ovary), or NSO cell.
12. The method of claim 1 , wherein the fusion protein is in a composition comprising a pharmaceutically acceptable carrier.
13. The method of claim 1 , wherein said human interferon alpha polypeptide is a human interferon alpha-2b polypeptide.
14. The method of claim 13 , wherein the human interferon alpha-2b polypeptide is fused to a human serum albumin protein consisting of the amino acid sequence of SEQ ID NO:18.
15. The method of claim 1 , wherein said method is for treating Hepatitis.
16. The method of claim 15 , wherein said Hepatitis is Hepatitis C.
17. The method of claim 15 , wherein said Hepatitis is Hepatitis B.
18. The method of claim 1 , wherein said method is for treating Cancer.
19. The method of claim 1 , wherein said method is for treating HIV.