Methods and devices for analyte detection
Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
1. A method, comprising:
resolving in a location of a fluid path one or more analytes in a sample disposed within the fluid path based on the size of the one or more analytes;
immobilizing at least one of the one or more analytes in the location of the fluid path;
after the immobilizing, conveying detection reagents through the fluid path; and
detecting at least one of the immobilized analytes by measuring a signal generated by the detection reagents in the location of the fluid path.
2. The method according to claim 1 , wherein said one or more analytes comprise proteins.
3. The method according to claim 1 , wherein said sample further comprises a standard.
4. The method according to claim 1 , wherein said sample is introduced into the fluid path by hydrodynamics.
5. The method according to claim 1 , wherein said sample is introduced into the fluid path by electrokinetic injection.
6. The method according to claim 1 , wherein said fluid path is comprised of one or more capillaries.
7. The method according to claim 1 , wherein said one or more analytes are resolved by electrophoresis.
8. The method according to claim 1 , wherein said fluid path comprises a sieving matrix.
9. The method according to claim 1 , wherein said fluid path comprises a detergent.
10. The method according to claim 1 , wherein said immobilization is achieved by photoactivated chemistry.
11. The method according to claim 1 wherein said detection reagents are comprised of antibodies.
12. The method of claim 1 , wherein the resolving includes resolving the one or more analytes prior to the immobilizing to enable the detection of a physical parameter of the one or more analytes.
13. The method of claim 1 , wherein the immobilizing is non-specific to any analyte from the one or more analytes.