Elastomeric device for cell seeding on the bottom of a filter
View Patent ↗An article is provided herein for use in seeding cells on at least one filter extending across at least one well of an assay device. The article includes an elastomeric body having spaced-apart first and second surfaces, and at least one channel extending between, and through, the first and second surfaces. The channel is formed to sealingly, and detachably, engage an outer surface of the well with the filter being at least partially encompassed by the channel. Advantageously, with the subject invention, a cell monolayer can be formed on the exterior surface of the filter. The assay device may be a multiwell plate, an insert plate, a column, a test tube, or a pipette.
1. A method for seeding cells on at least one filter extending across at least one well of an assay device, said method comprising:
providing an assay device with at least one well with at least one filter extending across said at least one well;
providing a body having spaced-apart first and second surfaces, and at least one channel extending between, and through, said first and second surfaces; and,
disposing said body onto said well with said channel sealingly engaging said well at a location spaced from said filter and said filter being at least partially encompassed by said channel,
wherein said channel is tapered so as to converge from said first surface to said second surface, said well extending into said channel through said second surface with said body being disposed on said well.
2. A method as in claim 1 , wherein said filter includes an interior surface facing the interior of said well and an exterior surface facing away from the interior of said well, said exterior surface of said filter being encompassed by said channel.
3. A method as in claim 2 , further comprising the step of directing said exterior surface of said filter to face gravitationally upwardly before the step of disposing said body onto said well.
4. A method as in claim 3 , further comprising the step of seeding cells on said interior surface of said filter.
5. A method as in claim 2 , further comprising the step of seeding cells on said interior surface of said filter.
6. A method as in claim 1 , wherein said body is elastomeric.
7. A method as in claim 1 , wherein said body is formed from polydimethylsiloxane.
8. A method as in claim 1 , further comprising the step of disposing at least one cell media solution in said channel after the step of disposing said body onto said well.
9. A method as in claim 8 , further comprising the step of allowing cell incubation after the step of disposing at least one cell media solution in said channel.
10. A method as in claim 9 , further comprising the step of detaching said body from said well after the step of allowing cell incubation.
11. A method as in claim 8 , further comprising the step of detaching said body from said well after the step of disposing at least one cell media solution in said channel.
12. A method as in claim 1 , wherein said assay device is selected from the group consisting of multiwell plates, insert plates, columns, test tubes, and pipettes.