Immunizing compositions and methods of use

1. A method for inducing the production of an antibody that specifically binds at least one SRP in an animal,

the method comprising administering to an animal an effective amount of a composition comprising:

at least two SRPs isolated from a gram negative microbe;

LPS at a concentration of no greater than about 10.0 EU/ml; and

a pharmaceutically acceptable carrier, wherein the composition induces in the animal antibody that specifically binds at least one SRP.

2. The method of claim 1 wherein the gram negative microbe is an enteropathogen.

3. The method of claim 1 wherein the gram negative microbe is a member of the family Enterobacteriaceae, family Vibrionaceae, family Pasteurellaceae, or family Pseudomonadaceae.

4. The method of claim 1 wherein the gram negative microbe is a member of the tribe Escherichieae or Salmonelleae.

5. The method of claim 1 wherein the gram negative microbe is Salmonella spp., Escherichia coli, Actinobacillus spp., Haemophilus spp., or Campylobacter spp.

6. The method of claim 1 wherein the at least two SRPs have molecular weights of between about 60 kDa and about 100 kDa as determined by sodium dodecyl-polyacrylamide gel electrophoresis.

7. The method of claim 1 further comprising at least one porin having a molecular weight of between about 30 kDa and about 43 kDa as determined by sodium dodecyl-polyacrylamide gel electrophoresis.

8. The method of claim 1 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a Bison, a companion animal, or a member of the family Ceridae.

9. The method of claim 8 wherein the companion animal is a horse.

10. The method of claim 1 wherein the animal is a bovine, and wherein the method further includes reducing somatic cell count, increasing milk production, decreasing fecal shedding, treating intestinal colonization, or increasing body weight in the bovine.

11. A method for inducing the production of an antibody that specifically binds at least one SRP in an animal,

the method comprising administering to an animal an effective amount of a siderophore receptor polypeptide (SRP) composition comprising:

at least two SRPs isolated from a gram negative microbe;

lipopolysaccharide (LPS) at a concentration of no greater than the concentration of LPS in a reference composition comprising: the at least two siderophore receptor polypeptides isolated from the gram negative microbe and lipopolysaccharide; wherein the reference composition is produced by a process comprising:

providing the gram negative microbe;

disrupting the gram negative microbe in a buffer;

solubilizing the disrupted gram negative microbe for greater than about 24 hours in a solution comprising sarcosine to result in solubilized and insoluble cellular material, wherein a ratio of the sarcosine to gram weight of disrupted gram negative microbe is between about 0.8 gram sarcosine per about 4.5 grams of disrupted gram negative microbe and about 1.2 grams sarcosine per about 4.5 grams of disrupted gram negative microbe; and

isolating molecules of the gram negative microbe, wherein the isolated molecules comprise the at least two SRPs and LPS; and

a pharmaceutically acceptable carrier, wherein the composition induces in the animal antibody that specifically binds at least one SRP.

12. The method of claim 11 wherein the gram negative microbe is an enteropathogen.

13. The method of claim 11 wherein the gram negative microbe is a member of the family Enterobacteriaceae, family Vibrionaceae, family Pasteurellaceae, or family Pseudomonadaceae.

14. The method of claim 11 wherein the gram negative microbe is a member of the tribe Escherichieae or Salmonelleae.

15. The method of claim 11 wherein the gram negative microbe is Salmonella spp., Escherichia coli, Actinobacillus spp., Haemophilus spp., or Campylobacter spp.

16. The method of claim 11 wherein the at least two SRPs have molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

17. The method of claim 11 wherein the SRP composition further comprises at least one porin having a molecular weight of between about 30 kDa and about 43 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

18. The method of claim 11 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a Bison, a companion animal, or a member of the family Ceridae.

19. The method of claim 18 wherein the companion animal is a horse.

20. The method of claim 11 wherein the animal is a bovine, and wherein the method further includes reducing somatic cell count, increasing milk production, decreasing fecal shedding, treating intestinal colonization, or increasing body weight in the bovine.

21. The method of claim 3 wherein the member of the family Enterobacteriaceae is Shigella spp., Proteus spp., Klebsiella spp., Serratia spp., or Yersinia spp.

22. The method of claim 3 wherein the member of the family Vibrionaceae is Vibrio cholerae.

23. The method of claim 3 wherein the member of the family Pasteurellaceae is Pasturella spp.

24. The method of claim 23 wherein the Pasturella spp. is Pasturella multocida or Pasteurella haemolytica.

25. The method of claim 3 wherein the member of the family Pseudomonadaceae is Pseudomonas spp.

26. The method of claim 25 wherein the Pseudomonas spp. is Pseudomonas aeruginosa.

27. The method of claim 1 wherein the gram negative microbe is a first gram negative microbe, wherein the antibody specifically binds an SRP present on the surface of a second gram negative microbe.

28. The method of claim 27 wherein the second gram negative microbe is an enteropathogen.

29. The method of claim 27 wherein the second gram negative microbe is a member of the family Enterobacteriaceae, family Vibrionaceae, family Pasteurellaceae, or family Pseudomonadaceae.

30. The method of claim 29 wherein the member of the family Enterobacteriaceae is Shigella spp., Proteus spp., Klebsiella spp., Serratia spp., or Yersinia spp.

31. The method of claim 29 wherein the member of the family Vibrionaceae is Vibrio cholerae.

32. The method of claim 29 wherein the member of the family Pasteurellaceae is Pasturella spp.

33. The method of claim 32 wherein the Pasturella spp. is Pasturella multocida or Pasteurella haemolytica.

34. The method of claim 29 wherein the member of the family Pseudomonadaceae is Pseudomonas spp.

35. The method of claim 34 wherein the Pseudomonas spp. is Pseudomonas aeruginosa.

36. The method of claim 27 wherein the first and second gram negative microbes are different species.

37. The method of claim 27 wherein the first and second gram negative microbes are different genera.

38. The method of claim 27 wherein the second gram negative microbe is a member of the tribe Escherichieae or Salmonelleae.

39. The method of claim 27 wherein the second gram negative microbe is Salmonella spp., Escherichia coli, Actinobacillus spp., Haemophilus spp., or Campylobacter spp.

40. The method of claim 1 wherein the animal has or is at risk of having a gram negative infection.

41. The method of claim 1 wherein the method further comprises collecting the antibody.

42. The method of claim 41 wherein the method further comprises administering the collected antibody to a second animal.

43. The method of claim 42 wherein the administering the collected antibody comprises administration of colostrum to the second animal.

44. The method of claim 41 wherein the collecting comprises collecting serum, plasma, blood, or colostrum.

45. The method of claim 1 wherein the method further comprises isolating the antibody.

46. The method of claim 13 wherein the member of the family Enterobacteriaceae is Shigella spp., Proteus spp., Klebsiella spp., Serratia spp., or Yersinia spp.

47. The method of claim 13 wherein the member of the family Vibrionaceae is Vibrio cholerae.

48. The method of claim 13 wherein the member of the family Pasteurellaceae is Pasturella spp.

49. The method of claim 48 wherein the Pasturella spp. is Pasturella multocida or Pasteurella haemolytica.

50. The method of claim 13 wherein the member of the family Pseudomonadaceae is Pseudomonas spp.

51. The method of claim 50 wherein the Pseudomonas spp. is Pseudomonas aeruginosa.

52. The method of claim 11 wherein the gram negative microbe is a first gram negative microbe, wherein the antibody specifically binds an SRP present on the surface of a second gram negative microbe.

53. The method of claim 52 wherein the second gram negative microbe is an enteropathogen.

54. The method of claim 52 wherein the second gram negative microbe is a member of the family Enterobacteriaceae, family Vibrionaceae, family Pasteurellaceae, or family Pseudomonadaceae.

55. The method of claim 54 wherein the member of the family Enterobacteriaceae is Shigella spp., Proteus spp., Klebsiella spp., Serratia spp., or Yersinia spp.

56. The method of claim 54 wherein the member of the family Vibrionaceae is Vibrio cholerae.

57. The method of claim 54 wherein the member of the family Pasteurellaceae is Pasturella spp.

58. The method of claim 57 wherein the Pasturella spp. is Pasturella multocida or Pasteurella haemolytica.

59. The method of claim 54 wherein the member of the family Pseudomonadaceae is Pseudomonas spp.

60. The method of claim 59 wherein the Pseudomonas spp. is Pseudomonas aeruginosa.

61. The method of claim 54 wherein the first and second gram negative microbes are different species.

62. The method of claim 54 wherein the first and second gram negative microbes are different genera.

63. The method of claim 54 wherein the second gram negative microbe is a member of the tribe Escherichieae or Salmonelleae.

64. The method of claim 54 wherein the second gram negative microbe is Salmonella spp., Escherichia coli, Actinobacillus spp., Haemophilus spp., or Campylobacter spp.

65. The method of claim 54 wherein the animal has or is at risk of having a gram negative infection.

66. The method of claim 54 wherein the method further comprises collecting the antibody.

67. The method of claim 66 wherein the method further comprises administering the collected antibody to a second animal.

68. The method of claim 66 wherein the administering the collected antibody comprises administration of colostrum to the second animal.

69. The method of claim 66 wherein the collecting comprises collecting serum, plasma, blood, or colostrum.

70. The method of claim 11 wherein the method further comprises isolating the antibody.