Optimized Fc variants and methods for their generation
The present invention relates to optimized Fc variants, methods for their generation, and antibodies and Fc fusions comprising optimized Fc variants.
1. A protein comprising an Fc variant of an IgG protein, wherein said Fc variant comprises an amino acid modification in the Fc region of said IgG protein at position 332, wherein said Fc variant has increased binding affinity to an FcyR receptor relative to an aglycosylated form of said IgG protein, wherein said Fc variant is aglycosylated at position 297 of the Fc region, and wherein numbering is according to the EU Index.
2. A protein according to claim 1 , wherein said modification is selected from the group consisting of: 332A, 332D, 332E, 332H, 332N, 332Q, 332T, and 332Y.
3. A protein comprising an Fc variant of an IgG protein, wherein said Fc variant comprises an amino acid modification in the Fc region of said IgG protein at position 332, wherein said Fc variant is aglycosylated at position 297 of the Fc region, and wherein numbering is according to the EU index.