Polymer end group detection
View Patent ↗Provided herein are processes of detecting and quantifying the number of polymer end groups in a sample. In particular instances provided herein are processes of detecting.
1. A process for diagnosing or determining the severity of abnormal glycosaminoglycan accumulation or a disorder associated with abnormal glycosaminoglycan degradation in an individual, the process comprising the steps of:
(a) quantifying with an analytical device, within or from within a test biological sample from the individual, the amount of a population of glycosaminoglycans that have been tagged with a detectable label at the reducing end of the glycosaminoglycan; and
(b) displaying or recording a quantification of the amount of the population of tagged glycosaminoglycans
whereby the quantification of the population of tagged glycosaminoglycans is utilized to determine the presence, identity and/or severity of the disease or condition.
2. The process of claim 1 , further comprising the step of:
(a) collecting from the individual a biological sample that comprises glycosaminoglycans; and
(b) tagging the reducing end of a representative portion of the glycosaminoglycans in the population of glycosaminoglycans within the biological sample with the detectable label to provide the population of tagged glycosaminoglycans.
3. The process of claim 1 , wherein the one or more different types of tagged glycosaminoglycans are one or more different tagged heparan sulfate fragments.
4. The process of claim 1 , wherein quantifying the amount of a population of glycosaminoglycans that have been tagged comprises separately quantifying the amount of a population of one or more different tagged chondroitin sulfate fragments, one or more different tagged dermatan sulfate fragments, one or more different tagged heparan sulfate fragments, one or more different tagged keratan sulfate fragments, one or more different tagged hyaluronan fragments, or a combination thereof.
5. The process of claim 1 , further comprising purifying the glycosaminoglycans from the test biological sample prior to tagging the reducing end of the glycosaminoglycans.
6. The process of claim 1 , wherein the detectable label is a mass label, affinity label, radiolabel, chromophore, or a fluorescent label.
7. The process of claim 1 , wherein the disorder is a disorder associated with abnormal glycosaminoglycan degradation and is a lysosomal storage disease.
8. The process of claim 7 , wherein the disorder associated with abnormal glycosaminoglycan degradation is mucopolysaccharidosis (MPS).
9. The process of claim 8 , wherein the mucopolysaccharidosis (MPS) is MPS I, MPS II, MPS IIIA, MPS IIIB, MPS IIIC, MPS IIID, MPS IVA, MPS IVB, MPS VI, MPS VII, MPS IX, or a combination thereof.
10. The process of claim 1 , wherein the quantification of the population of tagged glycosaminoglycans is utilized to monitor the treatment of disorders associated with the abnormal degradation, biosynthesis and / or accumulation of glycosaminoglycans (GAGs), or a disorder associated therewith.
11. The process of claim 1 , wherein the disorder associated with abnormal glycosaminoglycan accumulation is cancer.