Dermal fillers comprising silk fibroin hydrogels and uses thereof
The present specification provides compositions useful as dermal fillers and methods using such compositions to treat a condition of skin.
1. A dermal filler composition comprising a gel phase and a carrier phase that increases the fluidity of the gel phase, the gel phase including
a) first hydrogel particles comprising a substantially sericin-depleted silk fibroin; and
b) second hydrogel particles comprising a matrix polymer including an elastic protein;
wherein the first hydrogel particles has a protein structure that makes the hydrogel particles resist biodegradation.
2. The composition of claim 1 , wherein the silk fibroin hydrogel particles comprises about 1% (w/v) to about 10% (w/v) of silk fibroin.
3. The composition of claim 1 , wherein the elastic protein is an elastin.
4. The composition of claim 1 , wherein the silk fibroin hydrogel particles and elastic protein hydrogel particles have a cross-sectional area from about 0.1 μm 2 to about 1000 μm 2 .
5. The composition of claim 1 , wherein the silk fibroin hydrogel particles further comprises an amphiphilic peptide.
6. The composition of claim 1 , wherein the carrier phase is water, a saline solution, or a buffered solution.
7. The composition of claim 1 , wherein the carrier phase further comprises lidocaine.
8. A method of treating a soft tissue condition in an individual in need thereof, the method comprising the step of administering a composition of claim 1 into a skin region of the individual, wherein the administration improves the condition.
9. The method of claim 8 , wherein the soft tissue condition is a breast tissue condition, a facial tissue condition, a neck condition, a skin condition, an upper arm condition, a lower arm condition, a hand condition, a shoulder condition, a back condition, a torso including abdominal condition, a buttock condition, an upper leg condition, a lower leg condition including calf condition, a foot condition including plantar fat pad condition, an eye condition, a genital condition, or a condition effecting another body part, region or area.
10. The method of claim 9 , wherein the breast tissue condition is a breast imperfection, a breast defect, a breast augmentation, or a breast reconstruction.
11. The method of claim 9 , wherein the facial tissue condition is a facial imperfection, a facial defect, a facial augmentation, or a facial reconstruction.
12. The method of claim 9 , wherein the facial tissue condition is a dermal divot, a sunken cheek, a thin lip, a nasal imperfection or defect, a retro-orbital imperfection or defect, a facial fold, a facial line, a facial wrinkle, or other size, shape or contour imperfection or defect of the face.
13. The method of claim 9 , wherein the facial tissue condition is skin dehydration, a lack of skin elasticity, skin roughness, a lack of skin tautness, a skin stretch line or mark, or skin paleness.