Epidermal tissue promoter derived from potato for monocots
View Patent ↗The presently disclosed subject matter includes nucleic acid sequences and expression cassettes that contain regulatory sequences derived from Solanum tuberosum chitinase C genes, for expression of recombinant gene products in plants. The expression cassette drives high transgene expression in the epidermal (L1) cell layer.
1. An isolated nucleic acid sequence comprising SEQ ID NO: 1.
2. An expression cassette comprising SEQ ID NO: 1, operably linked to a heterologous gene.
3. The expression cassette of claim 2 , further comprising SEQ ID NO: 2.
4. The expression cassette of claim 2 further comprising a eukaryotic gene regulatory sequence.
5. The expression cassette of claim 2 wherein the heterologous gene is a gene for resistance to herbicides, pests, disease, or drought.
6. A plant, plant tissue, or plant cell comprising the expression cassette of claim 2 .
7. The plant, plant tissue, or plant cell of claim 6 , wherein the plant, plant cell, or plant tissue is monocotyledonous.
8. The plant, plant tissue, or plant cell of claim 7 , wherein the plant, plant cell, or plant tissue is a maize plant, plant cell, or plant tissue.
9. A method of expressing a heterologous gene comprising:
a. providing an expression cassette comprising SEQ ID NO: 1, operably linked to a heterologous gene, wherein the expression cassette is functional in a plant, plant tissue, or plant cell;
b. introducing said expression cassette into a suitable vector for plant transformation;
c. transforming vector into a plant, plant tissue, or plant cell; and
d. creating a plant, plant tissue, or plant cell comprising the expression cassette, wherein the heterologous gene is expressed.
10. The method of claim 9 wherein the heterologous gene is expressed in an epidermal cell layer of aerial plant tissue.
11. The method of claim 9 , wherein the plant, plant tissue, or plant cell, or a portion thereof is monocotyledonous.
12. The method of claim 11 , wherein the plant, plant tissue, plant cell, or a portion thereof is a maize plant, plant tissue, plant cell, or portion thereof.
13. A plant, plant tissue, or plant cell, made by the method of claim 9 , or a portion thereof comprising the expression cassette.
14. A progeny, seed, or grain produced by the plant, plant tissue, plant cell, or portion thereof of claim 13 , wherein the progeny, seed, or grain comprises the expression cassette.