Multisignal labeling reagents, and processes and uses therefor
The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.
1. A composition of matter comprising a nucleic acid strand or a complex of two or more nucleic acid strands wherein said strand or complex comprises (i) two or more labeled nucleotides or labeled nucleotide analogs, and (ii) one or more binding partners different from said labels, wherein said binding partner is attached to said strand or said complex through a linker.
2. The composition of claim 1 , wherein said strand or complex comprises one or more unlabeled nucleotide analogs.
3. The composition of claim 1 or 2 , wherein said nucleotide analogs are selected from the group consisting of abasic nucleotides, spacer groups and peptide nucleic acid subunits.
4. The composition of claim 1 , wherein said labels are selected from the group consisting of fluorescent compounds, phosphorescent compounds, chemiluminescent compounds, chelating compounds, electron dense compounds, magnetic compounds, intercalating compounds, energy transfer compounds, and any combination thereof.
5. The composition of claim 1 , wherein said binding partners are selected from the group consisting of ligand/receptor, hormone/receptor, biotin/avidin, biotin/streptavidin and antigen/antibody pairs.
6. The composition of claim 1 , wherein said linker is attached to said strand or complex on a nucleotide or nucleotide analog and said attachment is through the sugar or phosphate position of said nucleotide or nucleotide analog.
7. The composition of claim 1 , wherein said linker comprises one or more carbon, nitrogen, oxygen, phosphorus or sulfur atoms, or a combination thereof.
8. The composition of claim 1 , wherein said linker comprises one or more peptide bonds, alkyl chains, alkene groups, alkyl groups, aryl groups, conjugated systems, sugars or derivatives thereof.
9. The composition of claim 1 , wherein said nucleic acid strands are linear.
10. The composition of claim 1 , wherein said composition comprises a nucleic acid complex that is formed through base-pairing.
11. The composition of claim 1 , wherein said linker is attached to said strand or complex on a nucleotide or nucleotide analog and said attachment is through a sugar or an analog thereof.
12. The composition of claim 1 , wherein said linker is attached to said strand or complex on a nucleotide or nucleotide analog and said attachment is through a phosphate or an analog thereof.
13. The composition of claim 1 , wherein said nucleic acid strands are branched.
14. The composition of claim 1 , wherein said composition comprises a nucleic acid complex that is formed through triple strand formation.