Methods for fabricating isolated micro- and nano-structures using soft or imprint lithography
The presently disclosed subject matter describes the use of fluorinated elastomer-based materials, in particular perfluoropolyether (PFPE)-based materials, in high-resolution soft or imprint lithographic applications, such as micro- and nanoscale replica molding, and the first nano-contact molding of organic materials to generate high fidelity features using an elastomeric mold. Accordingly, the presently disclosed subject matter describes a method for producing free-standing, isolated nanostructures of any shape using soft or imprint lithography technique.
1. A plurality of monodisperse micro or nano sized particles, comprising:
an engineered shape wherein;
each of the particles of the plurality comprise, in cross-section, parallel lateral surfaces and parallel top and bottom surfaces;
wherein the size of each particle is less than about 10 micrometers in a broadest dimension; and
each particle of the plurality of particles comprises
(i) a therapeutically active polysaccharide, protein, small molecule drug, or nucleic acid; and
(ii) a biodegradable polymer comprising poly(lactic acid) or derivatives thereof.
2. The particles of claim 1 , wherein the engineered shape is selected from the group consisting of an angle, an edge, an arc, a vertex, and a point.
3. The particles of claim 1 , wherein the particles are physically stable such that the engineered shape is retained.
4. The particles of claim 1 , wherein the particles comprise a charge.
5. The particles of claim 1 , wherein the particles further comprise a material that has been treated.
6. The particles of claim 5 , wherein the treatment comprises solvent removal.
7. The particles of claim 1 , wherein the particles further comprise a degradable material.
8. The particles of claim 7 , wherein the material degrades in response to a stimulus.
9. The particles of claim 8 , wherein the stimulus is selected from the group consisting of pH, radiation, ionic strength, temperature, and alternating magnetic or electric fields.
10. The particles of claim 8 , wherein the response to the stimuli is selected from the group consisting of swelling, heating, and dissolving.
11. The particles of claim 1 , wherein the engineered shape is selected from the group consisting of a trapezoid, a cone, a rectangle, and an arrow.
12. The particles of claim 1 , wherein the engineered shape comprises a replication of a self-assembled structure.
13. The particles of claim 1 , wherein the broadest dimension is less than about 3 micrometers.
14. The particles of claim 1 , wherein the broadest dimension is less than about 1 micrometer.
15. The particles of claim 1 , wherein the broadest dimension is less than about 750 nanometers.
16. The particles of claim 1 , wherein the broadest dimension is less than about 500 nanometers.
17. The particles of claim 1 , wherein the broadest dimension is less than about 250 nanometers.
18. The particles of claim 1 , wherein the broadest dimension is less than about 200 nanometers.
19. The particles of claim 1 , wherein the broadest dimension is less than about 100 nanometers.
20. The particles of claim 1 , wherein the broadest dimension is less than about 50 nanometers.
21. The particles of claim 1 , further comprising a material selected from the group consisting of a polymer, a solution, a monomer, a plurality of monomers, a polymerization initiator, a polymerization catalyst, an inorganic precursor, a metal precursor, a pharmaceutical agent, a tag, a magnetic material, a paramagnetic material, a contrast agent, a ligand, a cell penetrating peptide, a porogen, a surfactant, a plurality of immiscible liquids, a solvent, a charged species, a poly(ethylene glycol), and a poly(ethylene glycol)-diacrylate.
22. The particles of claim 1 , wherein the particles further comprise a cell targeting peptide.
23. The particles of claim 1 , further comprising a material selected from the group consisting of surfactants and porogens.
24. The particles of claim 1 , further comprising at least one second particle, said second particle comprising a second material configured and dimensioned into said second particle, wherein said second particle is coupled with at least one of the monodisperse micro or nano sized particles.
25. The particles of claim 1 , further comprising a substrate coupled with the plurality of particles.
26. The particles of claim 1 , further comprising a structure configured and dimensioned on a surface of at least one of the particles.
27. The particles of claim 1 , further comprising a biological targeting agent or a biological recognition motif.
28. The particles of claim 27 , wherein the biological recognition motif comprises biotin or avidin.