Biomarkers associated with nephropathy
Use of urine biomarkers for diagnosing nephropathy, monitoring nephropathy progress, and assessing efficacy of a nephropathy treatment. These urine biomarkers include leukocyte-associated Ig-like receptor-2, alpha-1 acid glycoprotein, their fragments, and combinations thereof.
1. A method of assessing renal toxicity of an agent, comprising: obtaining a plurality of urine samples from a subject treated with an agent at various time points during treatment, determining in each of the urine samples the level of the fragment of leukocyte-associated Ig-like receptor-2 consisting of the sequence of SEQ ID NO:1, wherein the level of SEQ ID NO:1 is determined by mass spectrometry assay or an immune assay; and assessing renal toxicity of the agent based on a change in the level SEQ ID NO:2 in urine during the treatment, wherein an increase in level of SEQ ID NO:1 in the course of the treatment indicates that the agent is renal toxic.
2. The method of claim 1 , wherein the agent is selected from the group consisting of a compound, an herb product, and a food product.
3. The method of claim 1 , wherein the determining step is carried out by an immune assay selected from the group consisting of ELISA, Western blot, radioimmunoassay (RIA), fluorescent immunoassay (FIA), and luminescence immunoassay (LIA).
4. The method of claim 1 , wherein the subject is a human.
5. The method of claim 1 , wherein the subject is a laboratory animal.
6. The method of claim 1 , wherein the subject is free of proteinuria.