Fluorescent dyes
The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties.
1. A compound having the structure:
wherein X comprises an anion.
2. The compound of claim 1 , having the structure:
3. The compound of claim 1 , having the structure:
4. A method of identifying a specific organelle or region in a cell of interest, the method comprising
(a) incubating the cell of interest with the compound of claim 1 , and
(b) identifying the location of the organelle or region in the cell of interest by identifying the compound that stains the organelle or region.
5. The method of claim 4 , wherein the compound is
and the organelle or region is an endoplasmic reticulum.
6. The method of claim 4 , wherein the compound is
and the organelle or region is a mitrochondrion.
7. A target molecule comprising the compound of claim 1 .
8. The target molecule of claim 7 , wherein the target molecule is a nucleoside, a nucleotide, an oligonucleotide, a polynucleotide, a peptide nucleic acid, a protein, an oligopeptide, an enzyme, an antibody, a cytokine, avidin, streptavidin, digoxigenin, an oligosaccharide, a polysaccharide, a lipid, a liposome, a glycolipid, or a dye.
9. The target molecule of claim 7 , wherein the target molecule is a protein, an oligopeptide, a nucleotide, an oligonucleotide, or a polynucleotide.
10. A compound having the structure:
wherein X comprises an anion and wherein the compound also has at least one reactive group.
11. The compound of claim 10 , wherein said reactive group comprises a nucleophilic reactive group, an electrophilic reactive group, a terminal alkene, a terminal alkyne, a coordinate group or an alkylating agent.
12. The compound of claim 10 , wherein said reactive group comprises a nucleophilic reactive group comprising a thiol, amine or hydroxyl group.
13. The compound of claim 10 , wherein said reactive group comprises a electrophilic reactive group comprising an isocyanate, isothiocyanate, monochlorotriazine, dichlorotriazine, 4,6,-dichloro-1,3,5-triazines, mono- or di-halogen substituted pyridine, mono- or di-halogen substituted diazine, maleimide, haloacetamide, aziridine, sulfonyl halide, acid halide, hydroxysuccinimide ester, hydroxysulfosuccinimide ester, imido ester, hydrazine, azidonitrophenol, azide, 3-(2-pyridyl dithio)-proprionamide, glyoxal or aldehyde group.
14. A method of labeling a target molecule, the method comprising attaching the compound of claim 10 to the target molecule by means of the reactive group, thereby labeling the target molecule.
15. The method of claim 14 , wherein said reactive group comprises a nucleophilic reactive group, an electrophilic reactive group, a terminal alkene, a terminal alkyne, a coordinate group or an alkylating agent.
16. The method of claim 14 , wherein said reactive group comprises a thiol, an amine or a hydroxyl group.
17. The method of claim 14 , wherein said reactive group comprises an isocyanate, isothiocyanate, monochlorotriazine, dichlorotriazine, 4,6,-dichloro-1,3,5-triazines, mono- or di-halogen substituted pyridine, mono- or di-halogen substituted diazine, maleimide, haloacetamide, aziridine, sulfonyl halide, acid halide, hydroxysuccinimide ester, hydroxysulfosuccinimide ester, imido ester, hydrazine, azidonitrophenol, azide, 3-(2-pyridyl dithio)-proprionamide, glyoxal or aldehyde group.