Duplex oligonucleotide complexes and methods for gene silencing by RNA interference
Provided herein are duplex oligonucleotide complexes which can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent(s). The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue or organism.
1. A method for inhibiting expression of a target gene in a cell, the method comprising delivering to said cell a duplex oligonucleotide complex comprising:
a. a sense strand that is nineteen nucleotides in length, wherein nucleotides 1 and 2 and all C and all U nucleotides on said sense strand are 2′O-methyl modified and the sense strand has a 3′ end;
b. an antisense strand that is twenty-one nucleotides in length, wherein
i. all C nucleotides and all U nucleotides on said antisense strand are 2′F modified;
ii. the antisense strand has a 5′ end and a 3′ end and one mismatched nucleotide with the sense strand at position 14 within the antisense strand and the opposite nucleotide 6 on the sense strand, wherein all other nucleotides within the sense strand are complementary to nucleotides on the antisense strand; and
iii. the sense strand and the antisense strand form a duplex having a two nucleotide overhang at the 3′end of the antisense strand;
c. a cholesterol molecule attached to the 3′ end of the sense strand via a C5 linker molecule, wherein the cholesterol-linker-sense strand has the structure:
and
d. a phosphate group at the 5′ end of the antisense strand, wherein following said delivering the expression of a target protein is decreased.
2. The method of claim 1 , wherein the delivering is passive.
3. The method of claim 1 , wherein the two nucleotide overhang comprises a phosphorothioate linkage.
4. The method of claim 1 , wherein the two nucleotide overhang comprises a UU sequence.
5. A method for inhibiting expression of a target gene in a cell, the method comprising delivering to said cell a duplex oligonucleotide complex comprising:
a. a sense strand that is nineteen nucleotides in length, wherein nucleotides 1 and 2 and all C and all U nucleotides on said sense strand are 2′O-methyl modified and the sense strand has a 3′ end;
b. an antisense strand that is twenty-one nucleotides in length, wherein
i. all C nucleotides and all U nucleotides on said antisense strand are 2′F modified;
ii. the antisense strand has a 5′ end and a 3′ end and one mismatched nucleotide with the sense strand at position 13 within the antisense strand and the opposite nucleotide 7 on the sense strand, wherein all other nucleotides within the sense strand are complementary to nucleotides on the antisense strand; and
iii. the sense strand and the antisense strand form a duplex having a two nucleotide overhang at the 3′ end of the antisense strand;
c. a cholesterol molecule attached to the 3′ end of the sense strand via a C5 linker molecule, wherein the cholesterol-linker-sense strand has the structure:
and
d. a phosphate group at the 5′ end of the antisense strand, wherein following said delivering the expression of a target protein is decreased.
6. The method of claim 5 , wherein the delivering is passive.
7. The method of claim 5 , wherein the two nucleotide overhang comprises a phosphorothioate linkage.
8. The method of claim 5 , wherein the two nucleotide overhang comprises a UU sequence.
9. The method according to claim 1 , wherein the opposite nucleotide 6 on the sense strand is the same nucleotide that occurs at position 14 of the antisense strand.
10. The method according to claim 5 , wherein the opposite nucleotide 7 on the sense strand is the same nucleotide that occurs at position 13 of the antisense strand.
11. The method according to claim 1 , wherein all nucleotides in the sense strand and in the antisense strand other than nucleotides 1 and 2 of the sense strand and all C nucleotides and all U nucleotides of the sense and antisense strands comprise a 2′-OH.
12. The method according to claim 5 , wherein all nucleotides in the sense strand and in the antisense strand other than nucleotides 1 and 2 of the sense strand and all C nucleotides and all U nucleotides of the sense and antisense strands comprise a 2′-OH.
13. The method according to claim 1 , wherein between about 40% and about 90% of the nucleotides of the sense strand and between about 40% and about 90% of the nucleotides of the antisense strand are chemically modified.
14. The method according to claim 5 , wherein between about 40% and about 90% of the nucleotides of the sense strand and between about 40% and about 90% of the nucleotides of the antisense strand are chemically modified.
15. The method of claim 1 , wherein said delivering to said cell is by reverse transfection.
16. The method of claim 5 , wherein said delivering to said cell is by reverse transfection.
17. The method of claim 1 , wherein said delivering to said cell is in vivo.
18. The method of claim 5 , wherein said delivering to said cell is in vivo.