Methods for distinguishing between lung squamous carcinoma and other non small cell lung cancers
View Patent ↗The present invention provides nucleic acid sequences that are used for identification, classification and diagnosis of specific types of nonsmall-cell lung cancers (NSCLC). The nucleic acid sequences can also be used for prognosis evaluation of a subject based on the expression pattern of a biological sample.
1. A method for distinguishing between lung squamous cell carcinoma and other Non Small Cell Lung Carcinoma (NSCLC), the method comprising:
obtaining a lung cancer sample from a subject; determining in said sample an expression level of a nucleic acid sequence of SEQ ID NO: 1; and comparing said expression level to an expression level of a nucleic acid sequence of SEQ ID NO: 1 in a Non Small Cell Lung Carcinoma (NSCLC) sample; whereby increased expression of SEQ ID NO: 1 in the lung cancer sample in comparison to the Non Small Cell Lung Carcinoma (NSCLC) sample indicates the presence of squamous cell carcinoma.
2. The method of claim 1 , wherein said other Non Small Cell Lung Carcinoma (NSCLC) is lung adenocarcinoma.
3. The method of claim 1 , wherein the method comprises determining the expression levels of at least two nucleic acid sequences.
4. The method of claim 1 , wherein the method further comprises combining one or more expression ratios of said nucleic acid sequences.
5. The method of claim 1 , wherein the expression levels are determined by a method selected from the group consisting of nucleic acid hybridization, nucleic acid amplification, and a combination thereof.
6. The method of claim 5 , wherein the nucleic acid hybridization is performed using a solid-phase nucleic acid biochip array or in situ hybridization.
7. The method of claim 5 where n the nucleic acid amplification method is real-time PCR.
8. The method of claim 7 , wherein the real-time PCR method comprises forward and reverse primers.
9. The method of claim 8 , wherein the forward primer comprises a sequence selected from the group consisting of any one of SEQ ID NOS: 7-9.
10. The method of claim 8 , wherein the real-time PCR method further comprises a probe.
11. The method of claim 10 , wherein the probe comprises a sequence selected. from the group consisting of any one of SEQ ID NOS: 10-12.