Methods of modulating mesenchymal stem cell differentiation
View Patent ↗The present disclosure includes compositions and methods for modulating the differentiation of cells having osteogenic differentiation potential (such as mesenchymal stem cells (MSCs)) towards the osteogenic fate, and for obtaining diagnostic and prognostic information relating to diseases and disorders characterized by defects in osteogenic differentiation. The compositions include miRNAs, rm′RNA mimics, miRNA inhibitors, and siRNAs.
1. A method of promoting mesenchymal stem cell (MSC) differentiation toward an osteogenic cell fate, the method comprising introducing into a MSC an effective amount of a composition comprising at least one differentiation promoting agent that is a mimic of miR-148b and an effective amount of an inhibitor of miR-489, wherein the inhibitor of miR-489 comprises a sequence that is the same as SEQ ID NO: 2, and the mimic of miR-148b comprises an antisense strand that comprises the sequence of SEQ ID NO: 6.