Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
View Patent ↗The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO 2 and/or H 2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO 2 and H 2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.
1. A non-naturally occurring microbial organism, comprising exogenous proteins conferring to said microorganism a pathway to convert methanol and CO 2 , CO or H 2 , or a combination thereof, to acetyl-CoA, said pathway comprising methanol-methyltransferase activity comprising methanol methyltransferase, corrinoid protein, and methyltetrahydrofolate:corrinoid protein methyltransferase; and acetyl-CoA synthase/carbon monoxide dehydrogenase activity comprising methyltetrahydrofolate:corrinoid protein methyltransferase, corrinoid iron-sulfur protein, nickel-protein assembly protein, ferredoxin, acetyl-CoA synthase, and carbon monoxide dehydrogenase.
2. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
3. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase.
4. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism further comprises hydrogenase.
5. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase and hydrogenase.
6. The non-naturally occurring microbial organism of claim 2 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase.
7. The non-naturally occurring microbial organism of claim 2 , wherein said non-naturally occurring microbial organism further comprises hydrogenase.
8. The non-naturally occurring microbial organism of claim 2 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase and hydrogenase.
9. A method for producing acetyl-CoA, comprising culturing the non-naturally occurring microbial organism of claim 1 under conditions and for a sufficient period of time to produce acetyl-CoA.
10. The method of claim 9 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase.
11. The method of claim 9 , wherein said non-naturally occurring microbial organism further comprises hydrogenase.
12. The method of claim 9 , wherein said non-naturally occurring microbial organism is cultured in the presence of nitrate.
13. The method of claim 9 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase and hydrogenase.
14. The method of claim 12 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase.
15. The method of claim 12 , wherein said non-naturally occurring microbial organism further comprises hydrogenase.
16. The method of claim 12 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase and hydrogenase.
17. The method of claim 9 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
18. The method of claim 17 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase.
19. The method of claim 17 , wherein said non-naturally occurring microbial organism further comprises hydrogenase.
20. The method of claim 17 , wherein said non-naturally occurring microbial organism further comprises pyruvate ferredoxin oxidoreductase and hydrogenase.