Polypeptides having colanic acid-degrading activity
The present disclosure generally relates to polypeptides having colanic acid-degrading activity and methods of using the same. Polynucleotides encoding such polypeptides are also described. The polypeptides may be used, for example, in processes for degrading colanic acid, processes for the removal of endotoxins from biological samples, and processes for purifying plasmid DNA.
1. A composition comprising a plasmid DNA preparation and a purified polypeptide having colanic acid-degrading activity comprising an amino acid sequence having at least 98% homology to SEQ ID NOS: 1 or 2.
2. The composition of claim 1 , therein the purified polypeptide having colanic acid-degrading activity comprising the amino acid sequence having at least 99% homology to SEQ ID NOS: 1 or 2.
3. The composition of claim 1 , therein the purified polypeptide having colanic acid-degrading activity comprising the amino acid sequence of SEQ ID NOS: 1 or 2.
4. A composition comprising a purified DNA and a polypeptide having colanic acid-degrading activity comprising an amino acid sequence having at least 98% homology to SEQ ID NOS: 1 or 2.
5. The composition of claim 4 , therein the purified polypeptide having colonic acid-degrading activity comprising the amino acid sequence having at least 99% homology to SEQ ID NOS: 1 or 2.
6. The composition of claim 4 , therein the purified polypeptide having colonic acid-degrading activity comprising the amino acid sequence of SEQ ID NOS: 1 or 2.