Bacterially derived intact minicells that encompass plasmid free functional nucleic acid for in vivo delivery to mammalian cells
Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation.
1. A method for formulating a minicell composition comprising (a) a plurality of intact, bacterially derived minicells, each minicell of said plurality encompassing plasmid-free regulatory RNA, and (b) a pharmaceutically acceptable carrier therefor, the method consisting essentially of co-incubating a plurality of intact, bacterially derived minicells with regulatory RNA in a saline buffer, wherein said regulatory RNA is selected from the group consisting of siRNA, miRNA and shRNA.
2. The method of claim 1 , wherein said co-incubation comprises gentle shaking.
3. The method of claim 1 , wherein said co-incubation lasts about 0.5 hour.
4. The method of claim 1 , wherein said co-incubation lasts about one hour.
5. The method of claim 1 , wherein said buffered saline is in gelatin form.
6. The method of claim 1 , wherein said buffer comprises a 1× phosphate buffer solution.
7. The method of claim 1 , wherein said co-incubation is conducted at a temperature of about 4° C. to about 37° C.
8. The method of claim 1 , wherein said co-incubation is conducted at a temperature of about 20° C. to about 30° C.
9. The method of claim 1 , wherein said co-incubation is conducted at a temperature of about 25° C.
10. The method of claim 1 , wherein said co-incubation is conducted at a temperature of about 37° C.
11. The method of claim 10 , wherein said co-incubation comprises about 10 10 minicells.
12. The method of claim 1 , wherein said co-incubation comprises about 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , 10 12 or 10 13 minicells.
13. The method of claim 1 , wherein said co-incubation comprises about 10 10 minicells.