Affinity resin
View Patent ↗A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.
1. An affinity resin comprising a copolymer of a compound represented by the formula:
with a crosslinking agent wherein the crosslinking agent is selected from the group consisting of glycerol dimethylacrylate and triethylene glycol dimethylacrylate,
wherein Yb is a hydrogen atom or an amino-group-protecting group.
2. A screening method for a target molecule, which interacts specifically with a ligand, which method comprises:
(i) immobilizing the ligand to the resin of claim 1 ,
(ii) contacting the ligand-immobilized resin obtained in (i), with a sample, which may or may not comprise the target molecule,
(iii) identifying and/or analyzing the molecule, which interacts specifically or does not interact specifically with the ligand, and
(iv) judging the molecule that interacts specifically with the ligand to be a target molecule.
3. A method of measuring a target molecule, which interacts specifically with a ligand in a sample, which method comprises:
(i) immobilizing the ligand to the resin of claim 1 ,
(ii) contacting the ligand-immobilized resin obtained in (i) with a sample,
(iii) measuring and/or analyzing the molecule, which interacts specifically or does not interact specifically with the ligand, and
(iv) measuring a target molecule, which interacts specifically with the ligand.