Tissue scaffold
A tissue scaffold includes a first film having a plurality of cell openings and a second film adjacent the first film and having a plurality of cell openings larger than the cell openings of the first film. The cell openings of the first film interconnect with the cell openings of the second film to define pathways extending through the first and second films.
1. A tissue scaffold comprising:
a first film comprising a plurality of cell openings; and
a second film adjacent the first film and comprising a plurality of cell openings offset in a predetermined arrangement from the cell openings of the first film;
wherein the cell openings of the first film interconnect with the cell openings of the second film to define pathways with interconnecting pores of controlled area extending from the first film to the second film; and
wherein the diameter of a majority of the pores is less than the diameter of the cell openings of the first and second films that define each pore.
2. The tissue scaffold of claim 1 , further comprising a plurality of delivery channels extending from the first film to the second film.
3. The tissue scaffold of claim 1 , wherein at least one of the first and second films further comprises alignment holes to align the cell openings of the first and second films when joined together.
4. The tissue scaffold of claim 1 , wherein at least one of the cell openings comprises a therapeutic agent and, optionally, at least one of the films is an oriented film.
5. The tissue scaffold of claim 1 , wherein the first and second films are attached together using a biocompatible adhesive.
6. The tissue scaffold of claim 1 , wherein the first and second films are substantially circular.
7. The tissue scaffold of claim 6 , wherein at least one of the first and second films comprises progressively larger cell openings along a radial direction to define a cell opening gradient.
8. The tissue scaffold of claim 1 , wherein at least one of the first and second films comprises a plurality of cell openings sized and configured to define a cell opening gradient along the film.
9. The tissue scaffold of claim 1 , wherein the first film comprises a first material and the second film comprises a second material, wherein the first material has a higher absorption rate than the second material.
10. The tissue scaffold of claim 1 , wherein in a first orientation of the first film with respect to the second film, the cell openings of the first film are aligned with the cell openings of the second film to define a first plurality of pathways and wherein in a second orientation of the first film with respect to the second film, the cell openings of the first film are substantially offset from the cell openings of the second film to define a second plurality of pathways.
11. The tissue scaffold of claim 1 , wherein the cell openings of the first and second films are sized and configured to define multiple cell opening gradients to establish pathways for preferential cell culturing.
12. The tissue scaffold of claim 1 , wherein the cell openings include a diameter between about 10 to about 10,000 microns.
13. The tissue scaffold of claim 1 , wherein at least one of the first and second films comprises attachment regions configured to receive surgical fastening elements.
14. The tissue scaffold of claim 1 , wherein at least one of the cell openings comprises a material to substantially suppress tissue adhesion and at least one of the cell openings comprises a material to substantially promote tissue adhesion.
15. The tissue scaffold of claim 1 , wherein the cell openings of the second film are offset from the cell openings of the first film to form a combination of closed cells and open cells.
16. The tissue scaffold of claim 1 , wherein the scaffold is greater than 750 microns thick.
17. The tissue scaffold of claim 1 , wherein the scaffold is greater than 0.5 inches thick.
18. A tissue scaffold comprising:
a first film including a first porosity; and
a second film joined to the first film and including a second porosity greater than the first porosity; and
a plurality of delivery channels extending through the first and second films in a predetermined arrangement;
wherein the first porosity and the second porosity define a controlled porosity gradient extending from the first to the second film to selectively promote cellular regeneration along the gradient; and
wherein the diameter of a majority of the delivery channels is less than the diameter of the cell openings of the first and second films that define each channel.
19. A tissue scaffold comprising:
a first film including a plurality of cell openings; and
a second film adjacent the first film, the second film including a plurality of cell openings and alignment holes;
wherein the alignment holes align the cell openings of the first film with the cell openings of the second film such that the cell openings of the first film interconnect with the cell openings of the second film to define pathways with interconnecting pores of controlled area extending from the first film to the second film; and
wherein the diameter of a majority of the pores is less than the diameter of the cell openings of the first and second films that define each pore.
20. The tissue scaffold of claim 19 , wherein at least one of the first and second films comprises a plurality of cell openings sized and configured to define a cell opening gradient along the film.
21. The tissue scaffold of claim 19 , wherein in a first orientation of the first film with respect to the second film, the cell openings of the first film are aligned with the cell openings of the second film to define a first plurality of pathways and wherein a second orientation of the first film with respect to the second film, the cell openings of the first film are substantially offset from the cell openings of the second film to define a second plurality of pathways with interconnecting pores of controlled area extending from the first film to the second film.
22. The tissue scaffold of claim 19 , wherein at least one of the first and second films comprises attachment regions configured to receive surgical fastening elements.
23. The tissue scaffold of claim 19 , wherein the cell openings of the second film are offset from the cell openings of the first film to form a combination of closed cells and open cells.
24. The tissue scaffold of claim 19 , wherein the scaffold is greater than 750 microns thick.
25. The tissue scaffold of claim 19 , wherein the scaffold is greater than 0.5 inches thick.