Plants with increased yield
The present invention disclosed herein provides a method for producing a plant with increased yield as compared to a corresponding wild type plant comprising increasing or generating one or more activities in a plant or a part thereof. The present invention further relates to nucleic acids enhancing or improving one or more traits of a transgenic plant, and cells, progenies, seeds and pollen derived from such plants or parts, as well as methods of making and methods of using such plant cell(s) or plant(s), progenies, seed(s) or pollen. Particularly, said improved trait(s) are manifested in an increased yield, preferably by improving one or more yield-related trait(s), e.g. low temperature tolerance.
1. A method for producing a transgenic plant with increased yield compared to a corresponding non-transformed wild type plant, comprising:
(a) transforming a plant cell, a plant cell nucleus, or a plant tissue with a nucleic acid selected from the group consisting of:
(i) a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 147;
(ii) a nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 148; and
(iii) a nucleic acid encoding a polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 148;
and
(b) regenerating a transgenic plant from said transformed plant cell, plant cell nucleus, or plant tissue with increased yield compared to a corresponding non-transformed wild type plant.
2. The method of claim 1 , further comprising selecting a transgenic plant having increased yield compared to a corresponding non-transformed wild type plant on the basis of said transgenic plant showing increased yield as compared to the non-transformed wild type plant.
3. The method of claim 1 , further comprising obtaining a seed or progeny from said transgenic plant, wherein said seed or progeny comprises said nucleic acid and has increased yield compared to a corresponding non-transformed wild type plant.
4. The method of claim 1 , wherein the transgenic plant exhibits increased yield compared to a corresponding non-transformed wild type plant under standard growth conditions.
5. The method of claim 1 , wherein the transgenic plant exhibits increased yield compared to a corresponding non-transformed wild type plant under stress conditions.
6. The method of claim 1 , wherein the nucleic acid comprises:
(a) the nucleotide sequence of SEQ ID NO: 147; or
(b) a nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 148.
7. The method of claim 1 , wherein the plant cell, plant cell nucleus, or plant tissue is obtained from a monocotyledonous plant or a dicotyledonous plant.
8. A transgenic plant obtained by the method of 1 , or a seed or progeny of said transgenic plant, wherein said plant, seed or progeny comprises said nucleic acid.
9. A nucleic acid construct comprising:
(a) a nucleic acid selected from the group consisting of:
(i) a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 147;
(ii) a nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 148; and
(iii) a nucleic acid encoding a polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 148;
and
(b) one or more heterologous regulatory sequences operably linked to the nucleic acid of (a),
wherein said one or more regulatory sequences comprise a plant promoter.
10. The nucleic acid construct of claim 9 , wherein the nucleic acid comprises:
(a) the nucleotide sequence of SEQ ID NO: 147; or
(b) a nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 148.
11. A vector comprising the nucleic acid construct of claim 9 .
12. A host cell transformed stably or transiently with the nucleic acid construct of claim 9 or a vector comprising said nucleic acid construct, wherein said host cell is a plant cell or a microorganism.
13. A transgenic plant, plant cell, plant tissue or plant part comprising the nucleic acid construct of claim 9 .
14. The transgenic plant, plant cell, plant tissue or plant part of claim 13 , wherein said transgenic plant, plant cell, plant tissue or plant part is obtained by transforming a plant, plant cell, plant tissue or plant part with said nucleic acid construct.
15. The transgenic plant of claim 13 , wherein said transgenic plant exhibits an improved yield-related trait compared to a corresponding non-transformed wild type plant.
16. The transgenic plant of claim 13 , wherein said transgenic plant exhibits an improved nutrient use efficiency and/or abiotic stress tolerance compared to a corresponding non-transformed wild type plant.
17. The transgenic plant of claim 13 , wherein said transgenic plant exhibits an increased tolerance to low temperature compared to a corresponding non-transformed wild type plant.
18. The transgenic plant of claim 13 , wherein said transgenic plant exhibits an increased harvestable yield compared to a corresponding non-transformed wild type plant.
19. The transgenic plant of claim 13 , wherein said transgenic plant exhibits an increased yield compared to a corresponding non-transformed wild type plant, wherein said increased yield is calculated on a per plant basis or in relation to a specific arable area.
20. The transgenic plant, plant cell, plant tissue or plant part of claim 13 , wherein said transgenic plant is a monocotyledonous plant or a dicotyledonous plant, and wherein said plant cell, plant tissue or plant part is obtained from a monocotyledonous plant or a dicotyledonous plant.
21. The transgenic plant, plant cell, plant tissue or plant part of claim 13 , wherein the plant is selected from the group consisting of corn (maize), wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, oil seed rape, including canola and winter oil seed rape, manihot , pepper, sunflower, flax, borage, safflower, linseed, primrose, rapeseed, turnip rape, tagetes, solanaceous plants comprising potato, tobacco, eggplant, tomato; Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass, forage crops and Arabidopsis thaliana.
22. The transgenic plant, plant cell, plant tissue or plant part of claim 13 , wherein the plant is selected from the group consisting of corn, soy, oil seed rape, canola, winter oil seed rape, cotton, wheat and rice.
23. A method for enhancing a yield-related trait in a plant compared to a corresponding control plant, comprising:
(a) transforming the nucleic acid construct of claim 9 into a plant, plant cell or plant part;
(b) selecting a transgenic plant having an enhanced yield-related trait compared to a corresponding non-transformed control plant.
24. The method of claim 23 , wherein the plant, plant cell or plant part is obtained from a monocotyledonous plant or a dicotyledonous plant.
25. The method of claim 23 , wherein the enhanced yield-related trait comprises increased yield, enhanced tolerance to abiotic environmental stress and/or increased nutrient use efficiency.
26. The method of claim 25 , wherein the enhanced tolerance to abiotic environmental stress comprises an increased drought tolerance, an increased low temperature tolerance, an increased heat tolerance and/or an increased salt stress tolerance compared to a corresponding non-transformed control plant.