Regulatory polynucleotides and uses thereof
The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants. Methods for expressing polynucleotides in a plant cell, plant tissue, or plants using the regulatory polynucleotide molecules disclosed herein are also provided.
1. A polynucleotide molecule comprising
SEQ ID NO: 112 operably linked to a heterologous transcribable polynucleotide molecule.
2. The polynucleotide molecule of claim 1 , wherein the polynucleotide molecule is capable of regulating constitutive transcription.
3. The polynucleotide molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule encodes a protein of agronomic interest.
4. The polynucleotide molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule is operably linked to a 3′ transcription termination polynucleotide molecule.
5. A transgenic host cell comprising the polynucleotide molecule of claim 1 .
6. The transgenic host cell of claim 5 , wherein the host cell is a plant cell.
7. A transgenic plant stably transformed with the polynucleotide molecule of claim 1 .
8. The transgenic plant of claim 7 , wherein the plant is selected from the group consisting of a monocotyledonous and a dicotyledonous plant.
9. The transgenic plant of claim 8 , wherein the plant is a monocotyledonous plant selected from the group consisting of wheat, corn, rice, turf grass, millet, sorghum, switchgrass, miscanthus, sugarcane, and Brachypodium.
10. The transgenic plant of claim 8 , wherein the plant is a dicotyledonous plant selected from the group consisting of soybean, cotton, canola, and potato.
11. A seed produced by the transgenic plant of claim 7 , wherein the seed comprises the polynucleotide molecule.
12. A method of directing expression of a transcribable polynucleotide molecule in a host cell comprising:
(a) introducing the polynucleotide molecule of claim 1 into a host cell to produce a transgenic host cell; and
(b) selecting a transgenic host cell exhibiting expression of the heterologous transcribable polynucleotide molecule.
13. The method of claim 12 , wherein the heterologous transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA.
14. The method of claim 12 , wherein the host cell is a plant cell.
15. The method of claim 14 , further comprising regenerating a plant comprising the introduced polynucleotide molecule from the cell.
16. A method of directing expression of a transcribable polynucleotide molecule in a plant comprising:
(a) introducing the polynucleotide molecule of claim 1 into a plant cell;
(b) regenerating a plant from the plant cell; and
(c) selecting a transgenic plant exhibiting expression of the heterologous transcribable polynucleotide molecule.
17. The method of claim 16 , wherein the heterologous transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA.