Compositions and methods for establishing and/or maintaining pregnancy
Compositions comprising selenium (e.g., organic selenium (e.g., selenized yeast (e.g., SEL-PLEX))) and methods of using the same (e.g., as a therapeutic and/or prophylactic treatment) are provided. For example, compositions comprising selenium (e.g., organic selenium (e.g., selenized yeast (e.g., SEL-PLEX))) and methods of using the same are provided for treating and/or preventing one or more conditions (e.g., problems) disorders, and/or diseases related to establishing and/or maintaining a pregnancy. Compositions and methods of the invention find use in, among other things, research and clinical (e.g., preventative and therapeutic) applications.
1. A method of treating a female subject that is pregnant or attempting to become pregnant comprising:
a) identifying a female subject that will benefit from the treatment, comprising measuring a level of relaxin gene or gene product expression in said female subject, and identifying the female subject by determining whether the level of expression of relaxin gene or gene product is lower than a normal healthy control female subject, and
b) administering an effective amount of a composition comprising a dried, nonviable selenium-enriched yeast to said female subject to enhance-relaxin gene or gene product expression compared to a control female subject not administered said composition, wherein the total selenium content of said yeast comprises two percent or less inorganic selenium.
2. The method of claim 1 , wherein measuring relaxin gene or gene product expression comprises detecting nucleic acid expression and/or protein expression.
3. The method of claim 1 , wherein said method further comprises: c) measuring relaxin gene or gene product expression in said female subject after administration of said composition.
4. The method of claim 1 , wherein the female subject is identified as a subject selected from the group consisting of: a subject desiring reduction and/or elimination of the risk of perinatal morbidity and/or mortality; a subject desiring an increase in fetal growth in the subject; a subject desiring an increase in the duration of gestation in said subject; a subject desiring the promotion of connective tissue formation and/or rearrangement in the subject; a subject desiring promotion of proper formation of the pubis; a subject desiring a reduction in the risk and/or incidence of spontaneous abortion; a subject desiring a reduction in the risk and/or incidence of miscarriage; a subject desiring a reduction in the risk and/or incidence of stillbirth; and a subject desiring a reduction in the risk and/or incidence of birthing complications.
5. The method of claim 1 , wherein measuring relaxin gene expression comprises use of a nucleic acid probe.
6. A method of treating a female subject that is pregnant or attempting to become pregnant comprising:
a) identifying a female subject that will benefit from the treatment, wherein identifying comprises measuring the level of relaxin gene or gene product expression in said female subject, and identifying the female subject by determining whether the level of expression of relaxin gene or gene product is lower than a normal healthy control female subject; and
b) administering an effective amount of a composition comprising a dried, nonviable selenium-enriched yeast to said female subject to enhance a level of relaxin gene or gene product expression compared to a control subject not administered said composition, wherein the total selenium content of said yeast comprises two percent or less inorganic selenium;
c) measuring the level of relaxin gene or gene product expression in said female subject after administration of said composition; and
d) utilizing said level of relaxin gene or gene product expression after administration of said composition to determine a course of treatment selected from the group consisting of increasing the amount of said composition administered to said female subject, decreasing the amount of said composition administered to said female subject and terminating administration of said composition to said female subject.
7. The method of claim 6 , wherein relaxin gene product is relaxin protein.