Identification of tissue for debridement
View Patent ↗Provided are methods of determining whether a cell in a tissue site is viable or nonviable. Also provided are methods of debriding tissue from a tissue site. Further provided are kits comprising a compound that distinguishes between viable and nonviable cells and instructions for using the compound on a tissue site. Additionally, the use of a compound that distinguishes between viable and nonviable cells is provided, where the use is to determine whether a cell in a tissue site is viable or nonviable. Also provided is a use of a compound that distinguishes between viable and nonviable cells, where the use is for the manufacture of the above-described kit.
1. A method of determining the viability of a cell in a tissue site, the method comprising:
placing a solid sheet on the tissue site, the solid sheet comprising a compound configured for binding or reacting to a molecule secreted by nonviable tissue on the tissue site; and
determining whether the molecule is bound or has reacted to the compound, wherein areas of the sheet comprising the molecule bound to the compound are indicative of nonviable tissue.
2. The method of claim 1 , wherein the sheet is a polyhydroxyalkanoate.
3. The method of claim 1 , wherein the compound identifies a molecule that is depleted during cell death.
4. The method of claim 1 , wherein the compound comprises a dye capable of reacting with said molecule.
5. The method of claim 1 , wherein the molecule is a metabolite that is released from nonviable cells more than from viable cells.
6. The method of claim 5 , wherein the metabolite is selected from the group consisting of ATP, glucose, glycerol, NADPH and NADH.
7. The method of claim 1 , wherein the compound is selected from the group consisting of luciferase, luciferin, glucose oxidase, peroxidase, glycerol kinase, glycerol phosphate oxidase, alcohol dehydrogenase, a tetrazolium dye, phenazine methosulfate, ethidium bromide, and phenazine methosulfate.
8. The method of claim 1 , wherein the molecule is a nucleic acid.
9. The method of claim 1 , further comprising selectively debriding identified nonviable tissue.
10. The method of claim 1 , wherein the sheet is a starch film.
11. The method of claim 1 , wherein the sheet is a poly(D,L-lactide-co-glycolide).
12. The method of claim 1 , wherein the sheet is a polyglycolic acid.
13. The method of claim 1 , wherein the sheet is a poly-(L-lactic acid).