Process, tube and device for the preparation of wound healant composition

1. A method for the preparation of a wound healant composition, tissue healant composition, viscous gel, biological glue, mechanical support or filler comprising the steps of:

a) collecting whole blood in a container comprising hyaluronic acid, said container optionally further comprising a gel and/or an anticoagulant,

b) centrifuging said container to separate red blood cells from a supernatant comprising hyaluronic acid and enriched plasma,

c) optionally re-suspending or mixing said hyaluronic acid and the enriched plasma,

d) collecting the supernatant comprising said hyaluronic acid and said enriched plasma, and

e) optionally further mixing said hyaluronic acid and said enriched plasma.

2. The method according to claim 1 , wherein the method further comprises the following steps:

a) admixing said enriched plasma obtained under step 1(c), 1(d) and/or 1(e) with a coagulation activator or thrombin serum, and/or

b) admixing said enriched plasma obtained under step 1(c), 1(d) and/or 1(e) with at least one cell extract, cell composition, TCP, a bone substitute, chitosan, hyaluronic acid, cream, cream mask, fat cells, fat tissue, bone marrow concentrate, lubricin, cd-gelatin, botulinum toxin, calcium gluconate and/or stem cells,

wherein said cell extract is selected from an extract of keratinocytes, bone marrow cells, osteoblasts, chondrocytes, fibroblasts, periosteum or corneal cells, melanocytes, Langerhans cells, fat cells, muscle cells such as myoblasts and satellite cells, umbilical cord cells, mesenchymal stem cells (MSCs), preadipocytes, adipocytes, stem cells, periosteal cells, gingival cells, pre-endothelial cells, Schwann cells, ligament cells, tendon cells or pancreas islet cells.

3. The method according to claim 2 , wherein said centrifugation step is performed for a sufficient length of time to permit migration of red blood cells under said gel and/or migration of hyaluronic acid above said enriched plasma.

4. The method according to claim 2 , wherein said centrifugation step is performed at a force between about 1000 g and up to about 2000 g for a time selected from about 3 minutes up to about 7 minutes.

5. The method according to claim 2 , wherein:

a) said gel is a cell selector (thixotropic) gel;

b) said anticoagulant is sodium citrate;

c) said thrombin serum, whole blood, enriched plasma, and/or cell extract is autologous; and/or

d) said container is a tube.

6. The method according to claim 1 , wherein hyaluronic acid is present at the bottom of the container.

7. The method according to claim 1 , wherein the container comprises about 1 ml to about 2 ml hyaluronic acid.