Systems and methods for processing cells
The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heal seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
1. A tissue mincing tool comprising:
a compartment for a tissue sample;
a solid member moveable within the compartment;
a cutting surface at one end of the compartment; and
a sterile, sealed container,
wherein the compartment is arranged to position the tissue sample between the solid member and the cutting surface,
and further wherein the cutting surface separates the compartment from the sterile, sealed container, such that a tissue sample that is pressed, via the solid member, into contact with and through the cutting surface is deposited as a minced tissue within the sterile, sealed container.
2. The tissue mincing tool of claim 1 , wherein the cutting surface is dimensioned to mince the tissue sample into fragments having an average cross-section no greater than four square millimeters.
3. The tissue mincing tool of claim 1 , wherein the cutting surface is dimensioned to mince the tissue sample into fragments, and further comprising a second cutting surface for reducing an average cross-section of the fragments.
4. The tissue mincing tool of claim 1 , further comprising at least one mincer screen positioned in proximity to the cutting surface.
5. The tissue mincing tool of claim 4 , wherein the at least one mincer screen is moveable towards the tissue sample.
6. The tissue mincing tool of claim 1 , further comprising a suction cup for stabilizing the tissue mincing tool during operation thereof.
7. The tissue mincing tool of claim 1 , wherein the sterile, sealed container comprises at least one sealed access port permitting the sterile introduction of a fluid into the container.
8. The tissue mincing tool of claim 1 , wherein an interior surface of the compartment is threaded.
9. The tissue mincing tool of claim 1 , further comprising a gasket for sealing the compartment.
10. The tissue mincing tool of claim 1 , wherein a portion of the compartment near its end has a substantially constant cross-section.
11. The tissue mincing tool of claim 10 , wherein the solid member is shaped to mate with and to fill the portion of the compartment.
12. The tissue mincing tool of claim 1 , wherein the cutting surface is rotatable.
13. The tissue mincing tool of claim 1 , wherein the cutting surface is moveable towards the tissue sample.
14. The tissue mincing tool of claim 1 , further comprising a shaft crank for moving the cutting surface towards the tissue sample.
15. The tissue mincing tool of claim 1 , wherein the tissue mincing tool comprises a series of cutting surfaces at the one end of the compartment.