Method for the validatable inactivation of pathogens in a biological fluid by irradiation
View Patent ↗A validatable method for determining a photochemically effective dose for inactivating pathogens in a fluid sample is described herein. In particular, the instant invention covers methods for determining a photochemically effective dose sufficient to inactivate pathogens in a biological sample while leaving biologically active substances of interest unaffected. A batch irradiation reactor effective for inactivating pathogens in biological samples is also described.
1. A method for inactivating microorganisms in a biological fluid, the method comprising:
(a) determining a target light fluence effective to inactivate microorganisms in the biological fluid by:
(i) irradiating a dosimetric solution for calibration with a known incident light fluence of light comprising a first wavelength in a layer of the dosimetric solution having an optical path-length sufficiently thin to absorb less than 80% of the incident light comprising the first wavelength, thereby effecting a chemical change in the dosimetric solution measurable by a change in absorbance of the dosimetric solution at a second wavelength,
wherein the dosimetric solution matches the absorption coefficient of the biological fluid at the first wavelength, and further wherein the dosimetric solution is not a sample of the biologic fluid,
(ii) measuring the change in absorbance of the dosimetric solution at the second wavelength,
(iii) correlating the change in absorbance of the dosimetric solution to the known incident light fluence applied to the dosimetric solution to determine an effective light fluence applied to the dosimetric solution, and
(iv) correlating the effective light fluence applied to the dosimetric solution to a target light fluence effective to inactivate microorganisms in the biological fluid to determine the target light fluence effective to inactivate microorganisms in the biological fluid upon irradiation in a photoinactivation reactor; and
(b) irradiating the biological fluid in the photoinactivation reactor with the target light fluence of the light comprising the first wavelength.
2. The method according to claim 1 , wherein the optical path length of the dosimetric solution is sufficiently thin to absorb 50% or less of the incident light comprising the first wavelength.
3. The method according to claim 1 , further comprising monitoring the intensity of the one or more light sources during the irradiating (b) in order to determine a cumulative lamp intensity signal corresponding to the target light fluence applied to the biological fluid.
4. The method according to claim 1 , wherein the first wavelength of the light is in the UV range.
5. The method according to claim 1 , wherein the dosimetric solution comprises an agent or a combination of agents selected from the group consisting of alkali metal, alkaline earth metal and ammonium iodide, aqueous uridine phosphate, alkali metal, alkaline earth metal and ammonium salts of benzoic acid, and alkali metal, alkaline earth metal and ammonium peroxodisulfate, tert-butanol, and polyvinylpyrrolidone.
6. The method according to claim 1 , wherein the dosimetric solution comprises potassium iodide and potassium iodate.
7. The method according to claim 1 , wherein the dosimetric solution comprises potassium iodide, potassium iodate, and polyvinylpyrrolidone.
8. The method according to claim 1 , wherein the dosimetric solution comprises sodium benzoate.
9. The method according to claim 1 , wherein the dosimetric solution comprises a diluted potassium peroxodisulfate/tert-butanol actinometer.
10. The method according to claim 1 , wherein the biological fluid is contained in a stirred-batch photoinactivation reactor.
11. The method according to claim 1 , wherein said biological fluid comprises at least one additive to reduce damage and loss of biological activity of said fluid.
12. The method according to claim 1 , wherein said method is performed in conjunction with at least one other sterilization or microorganism inactivation method.
13. The method according to claim 1 , wherein said method is performed in conjunction with a solvent detergent treatment.
14. The method of claim 1 , wherein the dosimetric solution matches the absorption coefficient of the biological fluid at the first wavelength and the viscosity of the biological fluid.
15. The method of claim 1 , wherein the light comprising a first wavelength is a polychromatic light.
16. The method of claim 1 , wherein the target light fluence effective to inactivate microorganisms in the biological fluid is determined by biodosimetry.
17. A method for determining a target light fluence effective to inactivate microorganisms in a biological fluid, the method comprising:
(a) irradiating a dosimetric solution for calibration with a known incident light fluence of light comprising a first wavelength in a layer of the dosimetric solution having an optical path-length sufficiently thin to absorb less than 80% of the incident light comprising the first wavelength, thereby effecting a chemical change in the dosimetric solution measurable by a change in absorbance of the dosimetric solution at a second wavelength,
wherein the dosimetric solution matches the absorption coefficient of the biological fluid at the first wavelength, and further wherein the dosimetric solution is not a sample of the biologic fluid,
(b) measuring the change in absorbance of the dosimetric solution at the second wavelength,
(c) correlating the change in absorbance of the dosimetric solution to the known incident light fluence applied to the dosimetric solution to determine an effective light fluence applied to the dosimetric solution, and
(d) correlating the effective light fluence applied to the dosimetric solution to a target light fluence effective to inactivate microorganisms in the biological fluid to determine the target light fluence effective to inactivate microorganisms in the biological fluid upon irradiation in a photoinactivation reactor.
18. The method according to claim 17 , wherein the optical path length of the dosimetric solution is sufficiently thin to absorb 50% or less of the incident light comprising the first wavelength.
19. The method according to claim 17 , further comprising monitoring the intensity of the one or more light sources during the irradiating (b) in order to determine a cumulative lamp intensity signal corresponding to the target light fluence applied to the biological fluid.
20. The method according to claim 17 , wherein the first wavelength of the light is in the UV range.
21. The method according to claim 17 , wherein the dosimetric solution comprises an agent or a combination of agents selected from the group consisting of alkali metal, alkaline earth metal and ammonium iodide, aqueous uridine phosphate, alkali metal, alkaline earth metal and ammonium salts of benzoic acid, and alkali metal, alkaline earth metal and ammonium peroxodisulfate, tert-butanol, and polyvinylpyrrolidone.
22. The method according to claim 17 , wherein the dosimetric solution comprises a diluted potassium iodide and potassium iodate.
23. The method according to claim 17 , wherein the dosimetric solution comprises potassium iodide, potassium iodate, and polyvinylpyrrolidone.
24. The method according to claim 17 , wherein the dosimetric solution comprises sodium benzoate.
25. The method according to claim 17 , wherein the dosimetric solution comprises potassium peroxodisulfate and tert-butanol.
26. The method according to claim 17 , wherein said method is performed in conjunction with at least one other sterilization or microorganism inactivation method.
27. The method according to claim 17 , wherein said method is performed in conjunction with a solvent detergent treatment.
28. The method of claim 17 , wherein the dosimetric solution matches the absorption coefficient of the biological fluid at the first wavelength and the viscosity of the biological solution.
29. The method of claim 17 , wherein the light comprising a first wavelength is a polychromatic light.
30. The method of claim 17 , wherein the target light fluence effective to inactivate microorganisms in the biological fluid is determined by biodosimetry.