CD93 or use of soluble fragment thereof
The present invention relates to an anti-inflammatory composition using the antibody specifically binding to CD93 or its soluble fragment, and a diagnostic method and a diagnostic kit for inflammatory disease using CD93 or its soluble fragment specific antibody or aptamer.
1. A method for diagnosing rheumatoid arthritis comprising:
coating proteins from a biological sample obtained from synovial fluid and a control on a fixture;
inducing an antigen-antibody reaction by adding an antibody specifically binding to a CD93 soluble fragment to the above fixture;
detecting the antigen-antibody reaction product produced by the above antigen-antibody reaction by using a secondary antibody conjugate marker and chromogenic substrate solution;
comparing the detection results between the biological sample and the control; and
diagnosing rheumatoid arthritis or high risk of rheumatoid arthritis when CD93 soluble fragment is up-regulated in the biological sample, compared with the level in the control,
wherein the antigen-antibody reaction product is measured by a sandwich ELISA.
2. The method according to claim 1 , wherein the fixture is selected from the group consisting of nitrocellulose membrane, PVDF membrane (polyvinylidene difluoride membrane), 96-well plate made of polyvinyl resin or polystylene resin, and glass side glass.
3. The method according to claim 1 , wherein the secondary antibody conjugate marker is selected from the group consisting of HRP (horseradish peroxidase), alkaline phosphatase, colloid gold, FITC (poly L-lysine-fluorescein isothiocyanate), RITC (rhodamine-B-isothiocyanate), and dye.
4. The method according to claim 1 , wherein the chromogenic substrate solution is the one selected from the group consisting of TMB (3,3′,5,5′-tetramethyl bezidine), ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], and OPD (o-phenylenediamine).
5. The method according to claim 1 , wherein the soluble fragment is the 95 kDa ectodomain of CD93 protein released after cell culture.
6. The method according to claim 1 , wherein the soluble fragment has the amino acid sequence of SEQ ID NO:1.